Title: High efficiency of replication and expression of foreign genes in SV40-transformed human fibroblasts.
Abstract: Research Article1 December 1983free access High efficiency of replication and expression of foreign genes in SV40-transformed human fibroblasts. S. Boast S. Boast Search for more papers by this author G. La Mantia G. La Mantia Search for more papers by this author L. Lania L. Lania Search for more papers by this author F. Blasi F. Blasi Search for more papers by this author S. Boast S. Boast Search for more papers by this author G. La Mantia G. La Mantia Search for more papers by this author L. Lania L. Lania Search for more papers by this author F. Blasi F. Blasi Search for more papers by this author Author Information S. Boast, G. La Mantia, L. Lania and F. Blasi The EMBO Journal (1983)2:2327-2331https://doi.org/10.1002/j.1460-2075.1983.tb01742.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Human fibroblasts (HF) were transformed in vitro with origin-defective SV40 DNA (ori-) using the calcium phosphate co-precipitation technique. The SV40 ori- transformed human cells (HSF) were able to replicate efficiently a recombinant DNA molecule containing the ori sequence of SV40 DNA. Transfection of HFS with pTBC1, a recombinant pi vx plasmid containing the herpes simplex virus thymidine kinase (HSV-TK) gene and the ori SV40 sequences, results in high levels of TK mRNA of correct size. The pTBC1 plasmid does not appear to contain ‘poison’ sequences and can be efficiently re-established in Escherichia coli after replication in human cells. This host vector system may be of great usefulness in studying the expression of human genes in human cells. Previous ArticleNext Article Volume 2Issue 121 December 1983In this issue RelatedDetailsLoading ...