Title: Signature LIPID biomarker analysis for the quantitative analysis of environmental microbial ecology
Abstract: The signature lipid biomarker analysis (SLB) is a quantitative in situ analysis of microbial communities involving the extraction of both membrane lipids and DNA from slimes, muds, soils, ground water filter retentates, rhizospheres, bioreactor coupons and subsurface sediments followed by the fractionation of recoverable lipids into specific classes with identification by GUMS. The analysis allows for the estimation of the viable biomass, community composition, and nutritional status of the extant microbial consortia. The analysis is quantitative and is not limited by a necessity for isolation and culture of the organisms from the environmental matrices. The nature of the lipid extraction results in lysed cells which in turn allows for the recovery of purified DNA more suitable for gene probes analyses. Through the recovery of both phenotypic (PLFA) and genotypic (DNA) biomarkers from an individual sample, estimations of quantities and the metabolic status of individual species, families and/or classes of bacteria within the microbial community can be made. These analyses, based on microbial ecology, have been demonstrated to provide predictive value in bioprocess monitoring and bioremediation applications.
Publication Year: 1996
Publication Date: 1996-12-31
Language: en
Type: article
Access and Citation
Cited By Count: 12
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