Abstract:Part I. General Methods The Two-Hybrid System: A Personal View Stanley Fields and Paul L. Bartel Growth and Maintenance of Yeast Lawrence W. Bergman Media Formulations for Various Two-Hybrid Systems M...Part I. General Methods The Two-Hybrid System: A Personal View Stanley Fields and Paul L. Bartel Growth and Maintenance of Yeast Lawrence W. Bergman Media Formulations for Various Two-Hybrid Systems Michael Saghbini, Denise Hoekstra, and Jim Gautsch Yeast Two-Hybrid Vectors and Strains Philip James High-Efficiency Transformation of Plasmid DNA into Yeast Robin A. Woods and R. Daniel Gietz Qualitative and Quantitative Assessment of Interactions Monica M. Montano Strategies for Rescuing Plasmid DNA from Yeast Two-Hybrid Colonies, Alyson Byrd and Rene St-Arnaud Part II. False Positives Two-Hybrid System and False Positives: Approaches to Detection and Elimination Ilya G. Serebriiskii and Erica A. Golemis Confirming Yeast Two-Hybrid Protein Interactions Using In Vitro Glutathione-S-Transferase Pulldowns Dennis M. Kraichely and Paul N. MacDonald Two-Hybrid Interactions Confirmed by Coimmunoprecipitation of Epitope-Tagged Clones Louie Naumovski Part III. Applications Smad Interactors in Bone Morphogenetic Protein Signaling Xiangli Yang and Xu Cao Protein Interactions Important in Eukaryotic Translation Initiation Katsura Asano and Alan G. Hinnebusch Steroid Receptor and Ligand-Dependent Interaction with Coactivator Proteins Sergio A. Onate Interaction of Cellular Apoptosis Regulating Proteins with Adenovirus Anti-Apoptosis Protein E1B-19K Thirugnana Subramanian and G. Chinnadurai Part IV. Alternative Strategies Mammalian Two-Hybrid Assays: Analyzing Protein-Protein Interactions in the Transforming Growth Factor-_ Signaling Pathway Xin-Hua Feng and Rik Derynck One-Hybrid Systems for Detecting Protein-DNA Interactions Mary Kate Alexander, Brenda D. Bourns, and Virginia A.Zakian The Split-Hybrid System: Uncoding Multiprotein Networks and Defining Mutations That Affect Protein Interactions Phyllis S. Goldman, Anthony J. DeMaggio, Richard H. Goodman, and Merl F. Hoekstra Three-Hybrid Screens: Inducible Third-Party Systems Bjorn Sandrock, Franck Tirode, and Jean-Marc Egly Three-Hybrid Screens for RNA-Binding Proteins: Proteins Binding 3' End of Histone mRNA Zbigniew Dominski and William F. Marzluff Membrane Recruitment Systems for Analysis of Protein-Protein Interactions Ami AronheimRead More
Publication Year: 2001
Publication Date: 2001-01-01
Language: en
Type: book
Access and Citation
Cited By Count: 75
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot