Abstract: Basic Molecular Biology Techniques, Chooi-May Lai. Transient Transfection of Human Retinoblastoma Cells: Application to the Analysis of the Regulatory Regions of Photoreceptor-Specific Genes, Debora B. Farber, Leonid E. Lerner, and Andrea S. Viczian. In Situ Hybridization, Xian-Jie Yang. Immunocytochemical Studies of the Retina, Ann H. Milam. Antisense DNA Technology, Piroska Elizabeth Rakoczy. Inhibition of Gene Expression by Ribozymes, William W. Hauswirth, Lynn C. Shaw, Patrick O. Whalen, Jason J. Fritz, D. Alan White, and Alfred S. Lewin. Intraocular Delivery of Recombinant Virus, Fong-Qi Liang, Vibha Anand, Albert M. Maguire, and Jean Bennett. Preparation of Recombinant Adenoviruses, Karl G. Csaky. Generation of Recombinant Adeno-Associated Virus, Fabienne Rolling. Preparation of Recombinant Retroviruses, Xian-Jie Yang. Generation of Transgenic Mice for Studies of Ocular Development and Disease, Abha R. Gupta, Nadine S. Dejneka, Albert M. Maguire, and Jean Bennett. Generation of Knockout Animal Models, T. Michael Redmond. Mutation Analysis of Ocular Genes, Michael Danciger. Ophthalmologists' Perspective, Susan M. Downes, N. H. Victor Chong, and Alan C. Bird. Index.