Title: Genetic variation among wheat landraces with resistance to head scab revealed by RAPD markers.
Abstract: The genetic variation among 22 wheat landraces with resistant to head scab were estimated by RAPD markers.Fifty-seven arbitrary primers (10-mers) were used for the amplification of the genomic DNA, among which 48 primers (84.21 %) could produce 222 bands. Forty-five out of 48 primers (93.75 %) were polymorphic, resulting in 178 (80.18 %) polymorphic bands. One to 11 polymorphic bands could be amplified from each primer, with an average of 4.6 bands. Some special RAPD bands were only detected in certain wheat landraces. The mean GD based on RAPD makers among the 22 landraces were 0.314, ranging from 0.152 to 0.471. Based on the GD matrix, a dendrogram was constructed using the unweighted pair group method with arithmetic average (UPGMA). All the 22 scab-resistant wheat landraces could be distinguished by RAPD markers. Using the GD value of 0.33 as threshold, the 22 landraces could be clustered into 4 groups. It indicated that the genetic variation of the scab-resistant wheat landraces based on RAPD markers were correlated with the geographical distribution.
Publication Year: 2005
Publication Date: 2005-01-01
Language: en
Type: article
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