Title: Catecholamines inhibit early steps of lymphocyte activation
Abstract: In this study, the effect on extracellular adenosine concentration of direct activation of adenylyl cyclase by forskolin was investigated using rat cortical cultures. Forskolin evoked intracellular and extracellular cAMP accumulation as well as extracellular adenosine accumulation. The accumulation of adenosine in response to forskolin could be blocked by the cyclic nucleotide phosphodiesterase inhibitor 4-[(3-butoxy-4-methoxyphenyl)methyl]-2-imidazolidinone (RO 20-1724; 180 μM), but not isobutylmethylxanthine (100 μM). The accumulation of adenosine in response to forskolin could be blocked by the 5′-ectonucleotidase inhibitor guanosine 5′-monophosphate. These results demonstrate that forskolin can increase extracellular adenosine levels, and that this adenosine is ultimately derived from cAMP.
Publication Year: 1990
Publication Date: 1990-07-01
Language: en
Type: article
Indexed In: ['crossref']
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