Title: Markers of Platelet Activation and Granule Secretion
Abstract: Platelet activation results in a complex series of changes including a physical redistribution of receptors, changes in the molecular conformation of receptors, secretion of granule contents, development of a procoagulant surface, generation of platelet-derived microparticles, and formation of leukocyte-platelet aggregates. Each of these changes can potentially be used as a marker of platelet activation. Whole blood flow cytometry (1) is the method of choice for the measurement of all these changes, except the secretion of soluble molecules, which are usually measured by enzyme-linked immunosorbent assay (ELISA). Whole blood flow cytometry has many advantages, including: only minuscule volumes (∼5 µL) of blood are required; platelets are directly analyzed in their physiological milieu of whole blood; the minimal manipulation of samples prevents artifactual in vitro activation and potential loss of platelet subpopulations; both the activation state of circulating platelets and the reactivity of circulating platelets can be determined; and a spectrum of different activation-dependent changes can be determined. The specific methodological details of the use of flow cytometry to measure platelet activation are described elsewhere (1–3).
Publication Year: 2007
Publication Date: 2007-11-10
Language: en
Type: book-chapter
Indexed In: ['crossref']
Access and Citation
Cited By Count: 3
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