Title: Synthesis and in vitro antimicrobial evaluation of a new metronidazole derivative
Abstract: INTRODUCTION: For decades there has been active work on the synthesis, identification, and exploration of heterocyclic compounds. However, the imidazole nucleus is the main scaffold in molecules with various pharmacological properties such as antibacterial, antifungal, antineoplastic, antiviral, antidiabetic, etc. This work describes a synthesis of new metronidazole amide derivative by condensation of -(2-methyl-5-nitro-1Н-imidazole-1-yl)acetic acid with 2-(diethylamino)ethyl 4-aminobenzoate (procaine). The chemical structure of the new compound was confirmed by its IR and UV-VIS spectral data and was evaluated for in vitro potential antimicrobial activity against standard bacterial strains of Staphylococcus aureus ATCC 29213, Escherichia coli ATCC 25922, and Candida albicans ATCC 10231. AIM: The present study aimed to synthesize, characterize, and evaluate the potential antimicrobial activity of a new metronidazole amide compound against standard bacterial strains of Staphylococcus aureus ATCC 29213, Escherichia coli ATCC 25922, and Candida albicans 10231. MATERIALS AND METHODS: The new amide metronidazole derivative 2-(diethylamine) ethyl 4-(2-(2-methyl-5-nitro-1H-imidazole-1-yl)acetamide)benzoate was tested for in vitro antibacterial activity by the following described methods: cup-plate technique, minimum inhibitory concentration determination (MIC), and minimum bactericidal concentration (MBC) determination . RESULTS: A synthesis of a new metronidazole amide derivative was made by condensation of -(2-methyl-5-nitro-1Н-imidazole-1-yl)acetic acid with 2-(diethylamino)ethyl 4-aminobenzoate (procaine). The chemical structure of the new compound was confirmed by its IR and UV-VIS spectral data and was evaluated for in vitro potential antimicrobial activity against standard bacterial strains of Staphylococcus aureus ATCC 29213, Escherichia coli ATCC 25922, and Candida albicans ATCC 10231. CONCLUSION: The obtained data assess the same antimicrobial activity of the new compound and metronidazole against Staphylococcus aureus ATCC 29213, Escherichia coli ATCC 25922, and Candida albicans ATCC 10231. The highest sensitivity was observed against Candida albicans .