Abstract:Abstract Hydroxyl radicals have now been used for more than 15years to probe the structure of various macromolecules. They have also been widely used to footprint DNA-protein and RNA-protein complexes...Abstract Hydroxyl radicals have now been used for more than 15years to probe the structure of various macromolecules. They have also been widely used to footprint DNA-protein and RNA-protein complexes (1, 2). Hydroxyl radicals are w1y small, comparable in size to a water molecule, are most commonly generated from FeEDTA in solution by an innocuous and inexpensive procedure (see Protocols 2 and 3) <md are capable of cutting the DNA or RNA backbone at eve1y accessible nucleotide without sequence specificity (3-5). A footprinting reagent in which the .EDTA moiety is coupled to an intercalator can also be used to generate hydroxyl radicals (6). This reactant. methidium-propyl Fe-EDTA, binds to some preferred positions along the DNA, and consequently the pattern of DNA backbone cleavage is much less random than with Fee-EDTA alone.Read More
Publication Year: 2000
Publication Date: 2000-08-03
Language: en
Type: book-chapter
Indexed In: ['crossref']
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Cited By Count: 5
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