Get quick answers to your questions about the article from our AI researcher chatbot
{'id': 'https://openalex.org/W4387247730', 'doi': 'https://doi.org/10.1016/j.btre.2023.e00814', 'title': 'Transgenic expression in zebrafish embryos with an intact chorion by electroporation and microinjection', 'display_name': 'Transgenic expression in zebrafish embryos with an intact chorion by electroporation and microinjection', 'publication_year': 2023, 'publication_date': '2023-10-02', 'ids': {'openalex': 'https://openalex.org/W4387247730', 'doi': 'https://doi.org/10.1016/j.btre.2023.e00814', 'pmid': 'https://pubmed.ncbi.nlm.nih.gov/37840570'}, 'language': 'en', 'primary_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.1016/j.btre.2023.e00814', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S2764596916', 'display_name': 'Biotechnology Reports', 'issn_l': '2215-017X', 'issn': ['2215-017X'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320990', 'host_organization_name': 'Elsevier BV', 'host_organization_lineage': ['https://openalex.org/P4310320990'], 'host_organization_lineage_names': ['Elsevier BV'], 'type': 'journal'}, 'license': 'cc-by-nc-nd', 'license_id': 'https://openalex.org/licenses/cc-by-nc-nd', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': ['crossref', 'pubmed'], 'open_access': {'is_oa': True, 'oa_status': 'gold', 'oa_url': 'https://doi.org/10.1016/j.btre.2023.e00814', 'any_repository_has_fulltext': True}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5092312021', 'display_name': 'Nusrat Tazin', 'orcid': 'https://orcid.org/0000-0001-5531-7783'}, 'institutions': [{'id': 'https://openalex.org/I223532165', 'display_name': 'University of Utah', 'ror': 'https://ror.org/03r0ha626', 'country_code': 'US', 'type': 'education', 'lineage': ['https://openalex.org/I223532165']}], 'countries': ['US'], 'is_corresponding': True, 'raw_author_name': 'Nusrat Tazin', 'raw_affiliation_strings': ['Department of Electrical and Computer Engineering, University of Utah, Salt Lake City, Utah, United States of America'], 'affiliations': [{'raw_affiliation_string': 'Department of Electrical and Computer Engineering, University of Utah, Salt Lake City, Utah, United States of America', 'institution_ids': ['https://openalex.org/I223532165']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5055732792', 'display_name': 'Christopher Lambert', 'orcid': 'https://orcid.org/0000-0002-1877-099X'}, 'institutions': [{'id': 'https://openalex.org/I223532165', 'display_name': 'University of Utah', 'ror': 'https://ror.org/03r0ha626', 'country_code': 'US', 'type': 'education', 'lineage': ['https://openalex.org/I223532165']}], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Christopher Jordon Lambert', 'raw_affiliation_strings': ['Department of Mechanical Engineering, University of Utah, Salt Lake City, Utah, United States of America'], 'affiliations': [{'raw_affiliation_string': 'Department of Mechanical Engineering, University of Utah, Salt Lake City, Utah, United States of America', 'institution_ids': ['https://openalex.org/I223532165']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5106079790', 'display_name': 'Raheel Samuel', 'orcid': 'https://orcid.org/0000-0003-1882-2857'}, 'institutions': [{'id': 'https://openalex.org/I223532165', 'display_name': 'University of Utah', 'ror': 'https://ror.org/03r0ha626', 'country_code': 'US', 'type': 'education', 'lineage': ['https://openalex.org/I223532165']}], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Raheel Samuel', 'raw_affiliation_strings': ['Department of Mechanical Engineering, University of Utah, Salt Lake City, Utah, United States of America'], 'affiliations': [{'raw_affiliation_string': 'Department of Mechanical Engineering, University of Utah, Salt Lake City, Utah, United States of America', 'institution_ids': ['https://openalex.org/I223532165']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5045899573', 'display_name': 'Tamara J. Stevenson', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I223532165', 'display_name': 'University of Utah', 'ror': 'https://ror.org/03r0ha626', 'country_code': 'US', 'type': 'education', 'lineage': ['https://openalex.org/I223532165']}], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Tamara J. Stevenson', 'raw_affiliation_strings': ['Department of Pediatrics, University of Utah School of Medicine, Salt Lake City, Utah, United States of America'], 'affiliations': [{'raw_affiliation_string': 'Department of Pediatrics, University of Utah School of Medicine, Salt Lake City, Utah, United States of America', 'institution_ids': ['https://openalex.org/I223532165']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5065566020', 'display_name': 'Joshua L. Bonkowsky', 'orcid': 'https://orcid.org/0000-0001-8775-147X'}, 'institutions': [{'id': 'https://openalex.org/I223532165', 'display_name': 'University of Utah', 'ror': 'https://ror.org/03r0ha626', 'country_code': 'US', 'type': 'education', 'lineage': ['https://openalex.org/I223532165']}], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Joshua L. Bonkowsky', 'raw_affiliation_strings': ['Department of Pediatrics, University of Utah School of Medicine, Salt Lake City, Utah, United States of America'], 'affiliations': [{'raw_affiliation_string': 'Department of Pediatrics, University of Utah School of Medicine, Salt Lake City, Utah, United States of America', 'institution_ids': ['https://openalex.org/I223532165']}]}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5066534077', 'display_name': 'Bruce K. Gale', 'orcid': 'https://orcid.org/0000-0001-5843-3464'}, 'institutions': [{'id': 'https://openalex.org/I223532165', 'display_name': 'University of Utah', 'ror': 'https://ror.org/03r0ha626', 'country_code': 'US', 'type': 'education', 'lineage': ['https://openalex.org/I223532165']}], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Bruce K. Gale', 'raw_affiliation_strings': ['Department of Mechanical Engineering, University of Utah, Salt Lake City, Utah, United States of America'], 'affiliations': [{'raw_affiliation_string': 'Department of Mechanical Engineering, University of Utah, Salt Lake City, Utah, United States of America', 'institution_ids': ['https://openalex.org/I223532165']}]}], 'institution_assertions': [], 'countries_distinct_count': 1, 'institutions_distinct_count': 1, 'corresponding_author_ids': ['https://openalex.org/A5092312021'], 'corresponding_institution_ids': ['https://openalex.org/I223532165'], 'apc_list': {'value': 1850, 'currency': 'USD', 'value_usd': 1850, 'provenance': 'doaj'}, 'apc_paid': {'value': 1850, 'currency': 'USD', 'value_usd': 1850, 'provenance': 'doaj'}, 'fwci': 0.473, 'has_fulltext': False, 'cited_by_count': 1, 'citation_normalized_percentile': {'value': 0.638558, 'is_in_top_1_percent': False, 'is_in_top_10_percent': False}, 'cited_by_percentile_year': {'min': 67, 'max': 78}, 'biblio': {'volume': '40', 'issue': None, 'first_page': 'e00814', 'last_page': 'e00814'}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T12929', 'display_name': 'Animal Genetics and Reproduction', 'score': 0.9956, 'subfield': {'id': 'https://openalex.org/subfields/1311', 'display_name': 'Genetics'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, 'topics': [{'id': 'https://openalex.org/T12929', 'display_name': 'Animal Genetics and Reproduction', 'score': 0.9956, 'subfield': {'id': 'https://openalex.org/subfields/1311', 'display_name': 'Genetics'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T12789', 'display_name': 'Planarian Biology and Electrostimulation', 'score': 0.9813, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T10878', 'display_name': 'CRISPR and Genetic Engineering', 'score': 0.9797, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/transgenesis', 'display_name': 'Transgenesis', 'score': 0.96553934}], 'concepts': [{'id': 'https://openalex.org/C73247094', 'wikidata': 'https://www.wikidata.org/wiki/Q2790163', 'display_name': 'Transgenesis', 'level': 5, 'score': 0.96553934}, {'id': 'https://openalex.org/C186852380', 'wikidata': 'https://www.wikidata.org/wiki/Q1142521', 'display_name': 'Electroporation', 'level': 3, 'score': 0.938694}, {'id': 'https://openalex.org/C62525196', 'wikidata': 'https://www.wikidata.org/wiki/Q3311945', 'display_name': 'Microinjection', 'level': 2, 'score': 0.92814887}, {'id': 'https://openalex.org/C2776878037', 'wikidata': 'https://www.wikidata.org/wiki/Q169444', 'display_name': 'Zebrafish', 'level': 3, 'score': 0.8075309}, {'id': 'https://openalex.org/C102230213', 'wikidata': 'https://www.wikidata.org/wiki/Q430117', 'display_name': 'Transgene', 'level': 3, 'score': 0.75515366}, {'id': 'https://openalex.org/C196843134', 'wikidata': 'https://www.wikidata.org/wiki/Q33196', 'display_name': 'Embryo', 'level': 2, 'score': 0.6363789}, {'id': 'https://openalex.org/C95444343', 'wikidata': 'https://www.wikidata.org/wiki/Q7141', 'display_name': 'Cell biology', 'level': 1, 'score': 0.6172853}, {'id': 'https://openalex.org/C86803240', 'wikidata': 'https://www.wikidata.org/wiki/Q420', 'display_name': 'Biology', 'level': 0, 'score': 0.56854844}, {'id': 'https://openalex.org/C104317684', 'wikidata': 'https://www.wikidata.org/wiki/Q7187', 'display_name': 'Gene', 'level': 2, 'score': 0.2906341}, {'id': 'https://openalex.org/C54355233', 'wikidata': 'https://www.wikidata.org/wiki/Q7162', 'display_name': 'Genetics', 'level': 1, 'score': 0.26711047}, {'id': 'https://openalex.org/C2994278967', 'wikidata': 'https://www.wikidata.org/wiki/Q7314318', 'display_name': 'Reproductive technology', 'level': 4, 'score': 0.23939362}, {'id': 'https://openalex.org/C87073359', 'wikidata': 'https://www.wikidata.org/wiki/Q33196', 'display_name': 'Embryogenesis', 'level': 3, 'score': 0.21113679}], 'mesh': [], 'locations_count': 3, 'locations': [{'is_oa': True, 'landing_page_url': 'https://doi.org/10.1016/j.btre.2023.e00814', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S2764596916', 'display_name': 'Biotechnology Reports', 'issn_l': '2215-017X', 'issn': ['2215-017X'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320990', 'host_organization_name': 'Elsevier BV', 'host_organization_lineage': ['https://openalex.org/P4310320990'], 'host_organization_lineage_names': ['Elsevier BV'], 'type': 'journal'}, 'license': 'cc-by-nc-nd', 'license_id': 'https://openalex.org/licenses/cc-by-nc-nd', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, {'is_oa': True, 'landing_page_url': 'https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10569972', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S2764455111', 'display_name': 'PubMed Central', 'issn_l': None, 'issn': None, 'is_oa': True, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, {'is_oa': False, 'landing_page_url': 'https://pubmed.ncbi.nlm.nih.gov/37840570', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306525036', 'display_name': 'PubMed', 'issn_l': None, 'issn': None, 'is_oa': False, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.1016/j.btre.2023.e00814', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S2764596916', 'display_name': 'Biotechnology Reports', 'issn_l': '2215-017X', 'issn': ['2215-017X'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320990', 'host_organization_name': 'Elsevier BV', 'host_organization_lineage': ['https://openalex.org/P4310320990'], 'host_organization_lineage_names': ['Elsevier BV'], 'type': 'journal'}, 'license': 'cc-by-nc-nd', 'license_id': 'https://openalex.org/licenses/cc-by-nc-nd', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'sustainable_development_goals': [{'score': 0.54, 'display_name': 'Life below water', 'id': 'https://metadata.un.org/sdg/14'}], 'grants': [{'funder': 'https://openalex.org/F4320332161', 'funder_display_name': 'National Institutes of Health', 'award_id': None}], 'datasets': [], 'versions': [], 'referenced_works_count': 31, 'referenced_works': ['https://openalex.org/W1594199829', 'https://openalex.org/W1964353356', 'https://openalex.org/W1969454691', 'https://openalex.org/W1971723009', 'https://openalex.org/W1989100148', 'https://openalex.org/W2018110846', 'https://openalex.org/W2024375800', 'https://openalex.org/W2036275768', 'https://openalex.org/W2038072430', 'https://openalex.org/W2039080549', 'https://openalex.org/W2044914662', 'https://openalex.org/W2052649413', 'https://openalex.org/W2055202626', 'https://openalex.org/W2088926570', 'https://openalex.org/W2099688077', 'https://openalex.org/W2118007045', 'https://openalex.org/W2125349610', 'https://openalex.org/W2138789911', 'https://openalex.org/W2138838827', 'https://openalex.org/W2143263348', 'https://openalex.org/W2161533644', 'https://openalex.org/W2163919189', 'https://openalex.org/W2313900222', 'https://openalex.org/W2463979824', 'https://openalex.org/W2623660146', 'https://openalex.org/W3017013359', 'https://openalex.org/W3025085336', 'https://openalex.org/W3046966496', 'https://openalex.org/W3134929960', 'https://openalex.org/W4205867254', 'https://openalex.org/W4246926671'], 'related_works': ['https://openalex.org/W4387247730', 'https://openalex.org/W4239518686', 'https://openalex.org/W2410525919', 'https://openalex.org/W2409581157', 'https://openalex.org/W2138414905', 'https://openalex.org/W2066638490', 'https://openalex.org/W2064826992', 'https://openalex.org/W2046318795', 'https://openalex.org/W2009201430', 'https://openalex.org/W1985326250'], 'abstract_inverted_index': {'Electroporation': [0], 'is': [1, 14], 'regularly': [2], 'used': [3, 16], 'to': [4, 22], 'deliver': [5], 'agents': [6], 'into': [7, 56, 78], 'cells,': [8], 'including': [9], 'transgenic': [10], 'materials,': [11], 'but': [12], 'it': [13], 'not': [15], 'for': [17, 45, 73, 89, 120, 130], 'mutating': [18], 'zebrafish': [19, 49, 134], 'embryos': [20, 50, 135], 'due': [21], 'the': [23, 34, 38, 57, 60, 63, 71, 79, 82, 87, 101], 'lack': [24], 'of': [25, 122], 'suitable': [26], 'systems,': [27], 'information': [28], 'on': [29], 'appropriate': [30], 'operating': [31], 'parameters,': [32], 'and': [33, 62, 76, 84, 91, 105, 115], 'challenges': [35], 'posed': [36], 'by': [37, 53, 66], 'protective': [39], 'chorion.': [40], 'Here,': [41], 'a': [42, 111, 127, 142], 'novel': [43, 128], 'method': [44, 69, 129], 'gene': [46, 131], 'delivery': [47, 132], 'in': [48, 133, 141], 'was': [51], 'developed': [52, 110], 'combining': [54], 'microinjection': [55], 'space': [58, 80], 'between': [59, 81], 'chorion': [61, 74, 83], 'embryo': [64, 85], 'followed': [65], 'electroporation.': [67], 'This': [68], 'eliminates': [70, 86], 'need': [72, 88], 'removal': [75], 'injecting': [77], 'finding': [90], 'identifying': [92], 'key': [93], 'cell': [94], 'locations': [95], 'before': [96], 'performing': [97], 'an': [98], 'injection,': [99], 'making': [100], 'process': [102], 'much': [103], 'simpler': [104], 'more': [106], 'automatable.': [107], 'We': [108], 'also': [109], 'microfluidic': [112], 'electroporation': [113], 'system': [114], 'optimized': [116], 'electric': [117], 'pulse': [118], 'parameters': [119], 'transgenesis': [121, 145], 'embryos.': [123], 'The': [124], 'study': [125], 'provided': [126], 'that': [136], 'can': [137], 'be': [138], 'potentially': [139], 'implemented': [140], 'high': [143], 'throughput': [144], 'or': [146], 'mutagenesis': [147], 'system.': [148]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W4387247730', 'counts_by_year': [{'year': 2023, 'cited_by_count': 1}], 'updated_date': '2025-01-11T03:26:26.039868', 'created_date': '2023-10-03'}