Publication Information

{'id': 'https://openalex.org/W4386378159', 'doi': 'https://doi.org/10.1101/2023.08.31.555680', 'title': 'Improving split reporters of protein-protein interactions through orthology-based protein engineering', 'display_name': 'Improving split reporters of protein-protein interactions through orthology-based protein engineering', 'publication_year': 2023, 'publication_date': '2023-09-01', 'ids': {'openalex': 'https://openalex.org/W4386378159', 'doi': 'https://doi.org/10.1101/2023.08.31.555680'}, 'language': 'en', 'primary_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.1101/2023.08.31.555680', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306402567', 'display_name': 'bioRxiv (Cold Spring Harbor Laboratory)', 'issn_l': None, 'issn': None, 'is_oa': True, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I2750212522', 'host_organization_name': 'Cold Spring Harbor Laboratory', 'host_organization_lineage': ['https://openalex.org/I2750212522'], 'host_organization_lineage_names': ['Cold Spring Harbor Laboratory'], 'type': 'repository'}, 'license': 'cc-by-nc-nd', 'license_id': 'https://openalex.org/licenses/cc-by-nc-nd', 'version': 'submittedVersion', 'is_accepted': False, 'is_published': False}, 'type': 'preprint', 'type_crossref': 'posted-content', 'indexed_in': ['crossref'], 'open_access': {'is_oa': True, 'oa_status': 'green', 'oa_url': 'https://doi.org/10.1101/2023.08.31.555680', 'any_repository_has_fulltext': True}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5016625524', 'display_name': 'Louise-Marie Rakotoarison', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I4210141120', 'display_name': 'Sorbonne University Abu Dhabi', 'ror': 'https://ror.org/03e1ymy32', 'country_code': 'AE', 'type': 'education', 'lineage': ['https://openalex.org/I4210141120']}, {'id': 'https://openalex.org/I39804081', 'display_name': 'Sorbonne Université', 'ror': 'https://ror.org/02en5vm52', 'country_code': 'FR', 'type': 'education', 'lineage': ['https://openalex.org/I39804081']}], 'countries': ['AE', 'FR'], 'is_corresponding': False, 'raw_author_name': 'Louise-Marie Rakotoarison', 'raw_affiliation_strings': ['Sorbonne University'], 'affiliations': [{'raw_affiliation_string': 'Sorbonne University', 'institution_ids': ['https://openalex.org/I4210141120', 'https://openalex.org/I39804081']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5076676830', 'display_name': 'Alison G. Tebo', 'orcid': 'https://orcid.org/0000-0003-0788-5617'}, 'institutions': [{'id': 'https://openalex.org/I39804081', 'display_name': 'Sorbonne Université', 'ror': 'https://ror.org/02en5vm52', 'country_code': 'FR', 'type': 'education', 'lineage': ['https://openalex.org/I39804081']}, {'id': 'https://openalex.org/I4210141120', 'display_name': 'Sorbonne University Abu Dhabi', 'ror': 'https://ror.org/03e1ymy32', 'country_code': 'AE', 'type': 'education', 'lineage': ['https://openalex.org/I4210141120']}], 'countries': ['AE', 'FR'], 'is_corresponding': False, 'raw_author_name': 'Alison G. Tebo', 'raw_affiliation_strings': ['Sorbonne University'], 'affiliations': [{'raw_affiliation_string': 'Sorbonne University', 'institution_ids': ['https://openalex.org/I39804081', 'https://openalex.org/I4210141120']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5070738516', 'display_name': 'Dorothea Böken', 'orcid': 'https://orcid.org/0009-0008-8443-4469'}, 'institutions': [{'id': 'https://openalex.org/I4210141120', 'display_name': 'Sorbonne University Abu Dhabi', 'ror': 'https://ror.org/03e1ymy32', 'country_code': 'AE', 'type': 'education', 'lineage': ['https://openalex.org/I4210141120']}, {'id': 'https://openalex.org/I39804081', 'display_name': 'Sorbonne Université', 'ror': 'https://ror.org/02en5vm52', 'country_code': 'FR', 'type': 'education', 'lineage': ['https://openalex.org/I39804081']}], 'countries': ['AE', 'FR'], 'is_corresponding': False, 'raw_author_name': 'Dorothea Böken', 'raw_affiliation_strings': ['Sorbonne University'], 'affiliations': [{'raw_affiliation_string': 'Sorbonne University', 'institution_ids': ['https://openalex.org/I4210141120', 'https://openalex.org/I39804081']}]}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5074868501', 'display_name': 'Arnaud Gautier', 'orcid': 'https://orcid.org/0000-0003-1017-9879'}, 'institutions': [{'id': 'https://openalex.org/I4210141120', 'display_name': 'Sorbonne University Abu Dhabi', 'ror': 'https://ror.org/03e1ymy32', 'country_code': 'AE', 'type': 'education', 'lineage': ['https://openalex.org/I4210141120']}, {'id': 'https://openalex.org/I39804081', 'display_name': 'Sorbonne Université', 'ror': 'https://ror.org/02en5vm52', 'country_code': 'FR', 'type': 'education', 'lineage': ['https://openalex.org/I39804081']}], 'countries': ['AE', 'FR'], 'is_corresponding': True, 'raw_author_name': 'Arnaud Gautier', 'raw_affiliation_strings': ['Sorbonne University'], 'affiliations': [{'raw_affiliation_string': 'Sorbonne University', 'institution_ids': ['https://openalex.org/I4210141120', 'https://openalex.org/I39804081']}]}], 'institution_assertions': [], 'countries_distinct_count': 2, 'institutions_distinct_count': 2, 'corresponding_author_ids': ['https://openalex.org/A5074868501'], 'corresponding_institution_ids': ['https://openalex.org/I4210141120', 'https://openalex.org/I39804081'], 'apc_list': None, 'apc_paid': None, 'fwci': None, 'has_fulltext': False, 'cited_by_count': 5, 'citation_normalized_percentile': {'value': 0.999747, 'is_in_top_1_percent': True, 'is_in_top_10_percent': True}, 'cited_by_percentile_year': {'min': 90, 'max': 92}, 'biblio': {'volume': None, 'issue': None, 'first_page': None, 'last_page': None}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T13127', 'display_name': 'Biotin and Related Studies', 'score': 0.9989, 'subfield': {'id': 'https://openalex.org/subfields/1307', 'display_name': 'Cell Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, 'topics': [{'id': 'https://openalex.org/T13127', 'display_name': 'Biotin and Related Studies', 'score': 0.9989, 'subfield': {'id': 'https://openalex.org/subfields/1307', 'display_name': 'Cell Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T10540', 'display_name': 'Advanced Fluorescence Microscopy Techniques', 'score': 0.9985, 'subfield': {'id': 'https://openalex.org/subfields/1304', 'display_name': 'Biophysics'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T10617', 'display_name': 'Cellular transport and secretion', 'score': 0.9977, 'subfield': {'id': 'https://openalex.org/subfields/1307', 'display_name': 'Cell Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/bimolecular-fluorescence-complementation', 'display_name': 'Bimolecular fluorescence complementation', 'score': 0.8096969}, {'id': 'https://openalex.org/keywords/protein-fragment-complementation-assay', 'display_name': 'Protein-fragment complementation assay', 'score': 0.6970453}, {'id': 'https://openalex.org/keywords/fluorescent-protein', 'display_name': 'Fluorescent protein', 'score': 0.462309}, {'id': 'https://openalex.org/keywords/identification', 'display_name': 'Identification', 'score': 0.4352394}, {'id': 'https://openalex.org/keywords/protein-engineering', 'display_name': 'Protein Engineering', 'score': 0.43177396}], 'concepts': [{'id': 'https://openalex.org/C188082640', 'wikidata': 'https://www.wikidata.org/wiki/Q1780899', 'display_name': 'Complementation', 'level': 4, 'score': 0.9330242}, {'id': 'https://openalex.org/C123243835', 'wikidata': 'https://www.wikidata.org/wiki/Q863465', 'display_name': 'Bimolecular fluorescence complementation', 'level': 3, 'score': 0.8096969}, {'id': 'https://openalex.org/C115867849', 'wikidata': 'https://www.wikidata.org/wiki/Q753175', 'display_name': 'Protein-fragment complementation assay', 'level': 5, 'score': 0.6970453}, {'id': 'https://openalex.org/C125245961', 'wikidata': 'https://www.wikidata.org/wiki/Q221656', 'display_name': 'Brightness', 'level': 2, 'score': 0.6741399}, {'id': 'https://openalex.org/C70721500', 'wikidata': 'https://www.wikidata.org/wiki/Q177005', 'display_name': 'Computational biology', 'level': 1, 'score': 0.514477}, {'id': 'https://openalex.org/C3019336641', 'wikidata': 'https://www.wikidata.org/wiki/Q55643886', 'display_name': 'Fluorescent protein', 'level': 4, 'score': 0.462309}, {'id': 'https://openalex.org/C142613039', 'wikidata': 'https://www.wikidata.org/wiki/Q272983', 'display_name': 'Green fluorescent protein', 'level': 3, 'score': 0.43831837}, {'id': 'https://openalex.org/C116834253', 'wikidata': 'https://www.wikidata.org/wiki/Q2039217', 'display_name': 'Identification (biology)', 'level': 2, 'score': 0.4352394}, {'id': 'https://openalex.org/C147816474', 'wikidata': 'https://www.wikidata.org/wiki/Q169525', 'display_name': 'Protein engineering', 'level': 3, 'score': 0.43177396}, {'id': 'https://openalex.org/C186060115', 'wikidata': 'https://www.wikidata.org/wiki/Q30336093', 'display_name': 'Biological system', 'level': 1, 'score': 0.39921796}, {'id': 'https://openalex.org/C86803240', 'wikidata': 'https://www.wikidata.org/wiki/Q420', 'display_name': 'Biology', 'level': 0, 'score': 0.39285877}, {'id': 'https://openalex.org/C95444343', 'wikidata': 'https://www.wikidata.org/wiki/Q7141', 'display_name': 'Cell biology', 'level': 1, 'score': 0.35925066}, {'id': 'https://openalex.org/C12554922', 'wikidata': 'https://www.wikidata.org/wiki/Q7100', 'display_name': 'Biophysics', 'level': 1, 'score': 0.33201212}, {'id': 'https://openalex.org/C55493867', 'wikidata': 'https://www.wikidata.org/wiki/Q7094', 'display_name': 'Biochemistry', 'level': 1, 'score': 0.24754041}, {'id': 'https://openalex.org/C121332964', 'wikidata': 'https://www.wikidata.org/wiki/Q413', 'display_name': 'Physics', 'level': 0, 'score': 0.1843262}, {'id': 'https://openalex.org/C127716648', 'wikidata': 'https://www.wikidata.org/wiki/Q104053', 'display_name': 'Phenotype', 'level': 3, 'score': 0.13740504}, {'id': 'https://openalex.org/C120665830', 'wikidata': 'https://www.wikidata.org/wiki/Q14620', 'display_name': 'Optics', 'level': 1, 'score': 0.09909886}, {'id': 'https://openalex.org/C104317684', 'wikidata': 'https://www.wikidata.org/wiki/Q7187', 'display_name': 'Gene', 'level': 2, 'score': 0.09836736}, {'id': 'https://openalex.org/C59822182', 'wikidata': 'https://www.wikidata.org/wiki/Q441', 'display_name': 'Botany', 'level': 1, 'score': 0.071041524}, {'id': 'https://openalex.org/C181199279', 'wikidata': 'https://www.wikidata.org/wiki/Q8047', 'display_name': 'Enzyme', 'level': 2, 'score': 0.0}], 'mesh': [], 'locations_count': 2, 'locations': [{'is_oa': True, 'landing_page_url': 'https://doi.org/10.1101/2023.08.31.555680', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306402567', 'display_name': 'bioRxiv (Cold Spring Harbor Laboratory)', 'issn_l': None, 'issn': None, 'is_oa': True, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I2750212522', 'host_organization_name': 'Cold Spring Harbor Laboratory', 'host_organization_lineage': ['https://openalex.org/I2750212522'], 'host_organization_lineage_names': ['Cold Spring Harbor Laboratory'], 'type': 'repository'}, 'license': 'cc-by-nc-nd', 'license_id': 'https://openalex.org/licenses/cc-by-nc-nd', 'version': 'submittedVersion', 'is_accepted': False, 'is_published': False}, {'is_oa': True, 'landing_page_url': 'https://figshare.com/articles/journal_contribution/Improving_Split_Reporters_of_Protein_Protein_Interactions_through_Orthology-Based_Protein_Engineering/25111938', 'pdf_url': 'https://figshare.com/articles/journal_contribution/Improving_Split_Reporters_of_Protein_Protein_Interactions_through_Orthology-Based_Protein_Engineering/25111938/1/files/44312352.pdf', 'source': {'id': 'https://openalex.org/S4306400572', 'display_name': 'OPAL (Open@LaTrobe) (La Trobe University)', 'issn_l': None, 'issn': None, 'is_oa': True, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I196829312', 'host_organization_name': 'La Trobe University', 'host_organization_lineage': ['https://openalex.org/I196829312'], 'host_organization_lineage_names': ['La Trobe University'], 'type': 'repository'}, 'license': 'cc-by-nc', 'license_id': 'https://openalex.org/licenses/cc-by-nc', 'version': 'submittedVersion', 'is_accepted': False, 'is_published': False}], 'best_oa_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.1101/2023.08.31.555680', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306402567', 'display_name': 'bioRxiv (Cold Spring Harbor Laboratory)', 'issn_l': None, 'issn': None, 'is_oa': True, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I2750212522', 'host_organization_name': 'Cold Spring Harbor Laboratory', 'host_organization_lineage': ['https://openalex.org/I2750212522'], 'host_organization_lineage_names': ['Cold Spring Harbor Laboratory'], 'type': 'repository'}, 'license': 'cc-by-nc-nd', 'license_id': 'https://openalex.org/licenses/cc-by-nc-nd', 'version': 'submittedVersion', 'is_accepted': False, 'is_published': False}, 'sustainable_development_goals': [], 'grants': [], 'datasets': [], 'versions': [], 'referenced_works_count': 25, 'referenced_works': ['https://openalex.org/W1971439989', 'https://openalex.org/W2012935454', 'https://openalex.org/W2038447145', 'https://openalex.org/W2045006795', 'https://openalex.org/W2064894346', 'https://openalex.org/W2084766033', 'https://openalex.org/W2085369614', 'https://openalex.org/W2104870341', 'https://openalex.org/W2110221241', 'https://openalex.org/W2110399580', 'https://openalex.org/W2150028503', 'https://openalex.org/W2167427276', 'https://openalex.org/W2196795138', 'https://openalex.org/W2226712470', 'https://openalex.org/W2617804684', 'https://openalex.org/W2810720442', 'https://openalex.org/W2890247613', 'https://openalex.org/W2903410231', 'https://openalex.org/W2917896798', 'https://openalex.org/W2953795556', 'https://openalex.org/W3048007410', 'https://openalex.org/W3215400195', 'https://openalex.org/W3216309519', 'https://openalex.org/W4224036616', 'https://openalex.org/W4307032448'], 'related_works': ['https://openalex.org/W4386378159', 'https://openalex.org/W4384303339', 'https://openalex.org/W4378737118', 'https://openalex.org/W2999829375', 'https://openalex.org/W2977905319', 'https://openalex.org/W2317013365', 'https://openalex.org/W2165529326', 'https://openalex.org/W2154338693', 'https://openalex.org/W2095246184', 'https://openalex.org/W1979451155'], 'abstract_inverted_index': {'Summary': [0], 'Protein-protein': [1], 'interactions': [2], '(PPI)': [3], 'can': [4], 'be': [5], 'detected': [6], 'through': [7], 'selective': [8], 'complementation': [9, 101], 'of': [10, 15, 48, 65, 84, 95, 104, 109, 141], 'split': [11], 'fluorescent': [12, 24], 'reporters': [13], 'made': [14], 'two': [16], 'complementary': [17], 'fragments': [18], 'that': [19, 73], 'reassemble': [20], 'into': [21], 'a': [22, 34, 85, 92], 'functional': [23], 'reporter': [25, 37, 52], 'when': [26], 'in': [27, 143], 'close': [28], 'proximity.': [29], 'We': [30], 'previously': [31], 'introduced': [32], 'splitFAST,': [33], 'chemogenetic': [35], 'PPI': [36, 66, 127, 142], 'with': [38, 76, 87, 128], 'rapid': [39], 'and': [40, 58, 90, 107, 118, 131], 'reversible': [41], 'complementation.': [42], 'Here,': [43], 'we': [44], 'present': [45], 'the': [46, 82, 96, 100, 105, 139], 'engineering': [47, 71], 'RspA-splitFAST,': [49], 'an': [50, 68], 'improved': [51, 88, 119], 'displaying': [53], 'higher': [54, 59], 'brightness,': [55, 120], 'lower': [56], 'self-complementation': [57], 'dynamic': [60, 116], 'range': [61], 'for': [62], 'optimal': [63], 'monitoring': [64], 'using': [67], 'original': [69], 'protein': [70], 'strategy': [72], 'exploits': [74], 'proteins': [75], 'orthology': [77], 'relationships.': [78], 'Our': [79], 'study': [80, 126], 'allowed': [81], 'identification': [83], 'system': [86], 'properties': [89], 'enabled': [91], 'better': [93], 'understanding': [94], 'molecular': [97], 'features': [98], 'controlling': [99], 'properties.': [102], 'Because': [103], 'rapidity': [106], 'reversibility': [108], 'its': [110, 112], 'complementation,': [111], 'low': [113], 'self-complementation,': [114], 'high': [115, 129], 'range,': [117], 'RspA-splitFAST': [121], 'is': [122], 'well': [123], 'suited': [124], 'to': [125, 137], 'spatial': [130], 'temporal': [132], 'resolution,': [133], 'opening': [134], 'great': [135], 'prospects': [136], 'decipher': [138], 'role': [140], 'various': [144], 'biological': [145], 'contexts.': [146]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W4386378159', 'counts_by_year': [{'year': 2024, 'cited_by_count': 2}, {'year': 2023, 'cited_by_count': 3}], 'updated_date': '2024-12-17T17:06:07.685163', 'created_date': '2023-09-02'}