Abstract: Anthropological methods are used to study and estimate general parameters on unindentified individuals, significantly reducing the universe of suspects of unknown human remains.These methods are divided in two categories, metric and non-metric.Acknowledging craniometric variation is trivial to understand how crania can be used to estimate important biological parameters, such as sex, age and populational affinity (PA), formerly known as ancestry.The latter is seldom analyzed in Brazil, due to its high level of miscegenation.The objective of this work was to collect craniometric data from three main self-declared ethnicities in a southern Brazilian sample: Whites, Blacks and Pardos (Admixed).The sample was from a 1930s osteological collection from São Paulo, Brazil, consisted of 114 identified skulls.22 different craniometric measurements, described by Howells (1989), were done on each specimen.One-way ANOVA was used to assess statistical significance between mean differences, and Tukey's post-hoc test was used to pairwise analysis, when applicable.Only 9 measurements showed mean statistical difference between PAs.When significant, differences were mainly between the White and Black PA, whilst Admixed often presented an intermediate value, non-significant from others.PA estimation in Brazil is still fragile, by reason of the high admixing load of the population, and it is necessary to create a more representative anthropometric database of the Brazilian people, so already existing methods can be fairly reproduced.