Get quick answers to your questions about the article from our AI researcher chatbot
{'id': 'https://openalex.org/W4385329934', 'doi': 'https://doi.org/10.1186/s13007-023-01049-3', 'title': 'One pattern analysis (OPA) for the quantitative determination of protein interactions in plant cells', 'display_name': 'One pattern analysis (OPA) for the quantitative determination of protein interactions in plant cells', 'publication_year': 2023, 'publication_date': '2023-07-28', 'ids': {'openalex': 'https://openalex.org/W4385329934', 'doi': 'https://doi.org/10.1186/s13007-023-01049-3', 'pmid': 'https://pubmed.ncbi.nlm.nih.gov/37501124'}, 'language': 'en', 'primary_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.1186/s13007-023-01049-3', 'pdf_url': 'https://plantmethods.biomedcentral.com/counter/pdf/10.1186/s13007-023-01049-3', 'source': {'id': 'https://openalex.org/S184470774', 'display_name': 'Plant Methods', 'issn_l': '1746-4811', 'issn': ['1746-4811'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320256', 'host_organization_name': 'BioMed Central', 'host_organization_lineage': ['https://openalex.org/P4310319965', 'https://openalex.org/P4310320256'], 'host_organization_lineage_names': ['Springer Nature', 'BioMed Central'], 'type': 'journal'}, 'license': 'cc-by', 'license_id': 'https://openalex.org/licenses/cc-by', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': ['crossref', 'pubmed'], 'open_access': {'is_oa': True, 'oa_status': 'gold', 'oa_url': 'https://plantmethods.biomedcentral.com/counter/pdf/10.1186/s13007-023-01049-3', 'any_repository_has_fulltext': True}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5015541214', 'display_name': 'Jan Eric Maika', 'orcid': 'https://orcid.org/0009-0009-2110-0821'}, 'institutions': [{'id': 'https://openalex.org/I4210126213', 'display_name': 'Cluster of Excellence on Plant Sciences', 'ror': 'https://ror.org/034waa237', 'country_code': 'DE', 'type': 'facility', 'lineage': ['https://openalex.org/I1305996414', 'https://openalex.org/I149899117', 'https://openalex.org/I171892758', 'https://openalex.org/I180923762', 'https://openalex.org/I4210126213', 'https://openalex.org/I4210141639', 'https://openalex.org/I44260953']}, {'id': 'https://openalex.org/I44260953', 'display_name': 'Heinrich Heine University Düsseldorf', 'ror': 'https://ror.org/024z2rq82', 'country_code': 'DE', 'type': 'education', 'lineage': ['https://openalex.org/I44260953']}], 'countries': ['DE'], 'is_corresponding': False, 'raw_author_name': 'Jan Eric Maika', 'raw_affiliation_strings': ['Institute for Developmental Genetics and Cluster of Excellence on Plant Sciences, Heinrich Heine University, Düsseldorf, Germany'], 'affiliations': [{'raw_affiliation_string': 'Institute for Developmental Genetics and Cluster of Excellence on Plant Sciences, Heinrich Heine University, Düsseldorf, Germany', 'institution_ids': ['https://openalex.org/I4210126213', 'https://openalex.org/I44260953']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5028996018', 'display_name': 'Benedikt Krämer', 'orcid': 'https://orcid.org/0000-0002-1910-8156'}, 'institutions': [{'id': 'https://openalex.org/I4210104359', 'display_name': 'PicoQuant (Germany)', 'ror': 'https://ror.org/01a8nnm19', 'country_code': 'DE', 'type': 'company', 'lineage': ['https://openalex.org/I4210104359']}], 'countries': ['DE'], 'is_corresponding': False, 'raw_author_name': 'Benedikt Krämer', 'raw_affiliation_strings': ['PicoQuant GmbH, Berlin, Germany'], 'affiliations': [{'raw_affiliation_string': 'PicoQuant GmbH, Berlin, Germany', 'institution_ids': ['https://openalex.org/I4210104359']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5033021809', 'display_name': 'Vivien I. Strotmann', 'orcid': 'https://orcid.org/0000-0003-4822-6706'}, 'institutions': [{'id': 'https://openalex.org/I4210126213', 'display_name': 'Cluster of Excellence on Plant Sciences', 'ror': 'https://ror.org/034waa237', 'country_code': 'DE', 'type': 'facility', 'lineage': ['https://openalex.org/I1305996414', 'https://openalex.org/I149899117', 'https://openalex.org/I171892758', 'https://openalex.org/I180923762', 'https://openalex.org/I4210126213', 'https://openalex.org/I4210141639', 'https://openalex.org/I44260953']}, {'id': 'https://openalex.org/I44260953', 'display_name': 'Heinrich Heine University Düsseldorf', 'ror': 'https://ror.org/024z2rq82', 'country_code': 'DE', 'type': 'education', 'lineage': ['https://openalex.org/I44260953']}], 'countries': ['DE'], 'is_corresponding': False, 'raw_author_name': 'Vivien I. Strotmann', 'raw_affiliation_strings': ['Institute for Developmental Genetics and Cluster of Excellence on Plant Sciences, Heinrich Heine University, Düsseldorf, Germany'], 'affiliations': [{'raw_affiliation_string': 'Institute for Developmental Genetics and Cluster of Excellence on Plant Sciences, Heinrich Heine University, Düsseldorf, Germany', 'institution_ids': ['https://openalex.org/I4210126213', 'https://openalex.org/I44260953']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5031317899', 'display_name': 'Frank Wellmer', 'orcid': 'https://orcid.org/0000-0002-2095-0981'}, 'institutions': [{'id': 'https://openalex.org/I205274468', 'display_name': 'Trinity College Dublin', 'ror': 'https://ror.org/02tyrky19', 'country_code': 'IE', 'type': 'education', 'lineage': ['https://openalex.org/I205274468']}], 'countries': ['IE'], 'is_corresponding': False, 'raw_author_name': 'Frank Wellmer', 'raw_affiliation_strings': ['Smurfit Institute of Genetics, Trinity College Dublin, Dublin, Ireland'], 'affiliations': [{'raw_affiliation_string': 'Smurfit Institute of Genetics, Trinity College Dublin, Dublin, Ireland', 'institution_ids': ['https://openalex.org/I205274468']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5031557196', 'display_name': 'Stefanie Weidtkamp‐Peters', 'orcid': 'https://orcid.org/0000-0001-7734-3771'}, 'institutions': [{'id': 'https://openalex.org/I44260953', 'display_name': 'Heinrich Heine University Düsseldorf', 'ror': 'https://ror.org/024z2rq82', 'country_code': 'DE', 'type': 'education', 'lineage': ['https://openalex.org/I44260953']}], 'countries': ['DE'], 'is_corresponding': False, 'raw_author_name': 'Stefanie Weidtkamp-Peters', 'raw_affiliation_strings': ['Centre for Advanced Imaging, Heinrich Heine University, Düsseldorf, Germany'], 'affiliations': [{'raw_affiliation_string': 'Centre for Advanced Imaging, Heinrich Heine University, Düsseldorf, Germany', 'institution_ids': ['https://openalex.org/I44260953']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5088362029', 'display_name': 'Yvonne Stahl', 'orcid': 'https://orcid.org/0000-0002-7543-5186'}, 'institutions': [{'id': 'https://openalex.org/I4210126213', 'display_name': 'Cluster of Excellence on Plant Sciences', 'ror': 'https://ror.org/034waa237', 'country_code': 'DE', 'type': 'facility', 'lineage': ['https://openalex.org/I1305996414', 'https://openalex.org/I149899117', 'https://openalex.org/I171892758', 'https://openalex.org/I180923762', 'https://openalex.org/I4210126213', 'https://openalex.org/I4210141639', 'https://openalex.org/I44260953']}, {'id': 'https://openalex.org/I44260953', 'display_name': 'Heinrich Heine University Düsseldorf', 'ror': 'https://ror.org/024z2rq82', 'country_code': 'DE', 'type': 'education', 'lineage': ['https://openalex.org/I44260953']}], 'countries': ['DE'], 'is_corresponding': True, 'raw_author_name': 'Yvonne Stahl', 'raw_affiliation_strings': ['Institute for Developmental Genetics and Cluster of Excellence on Plant Sciences, Heinrich Heine University, Düsseldorf, Germany'], 'affiliations': [{'raw_affiliation_string': 'Institute for Developmental Genetics and Cluster of Excellence on Plant Sciences, Heinrich Heine University, Düsseldorf, Germany', 'institution_ids': ['https://openalex.org/I4210126213', 'https://openalex.org/I44260953']}]}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5001481767', 'display_name': 'Rüdiger Simon', 'orcid': 'https://orcid.org/0000-0002-1317-7716'}, 'institutions': [{'id': 'https://openalex.org/I4210126213', 'display_name': 'Cluster of Excellence on Plant Sciences', 'ror': 'https://ror.org/034waa237', 'country_code': 'DE', 'type': 'facility', 'lineage': ['https://openalex.org/I1305996414', 'https://openalex.org/I149899117', 'https://openalex.org/I171892758', 'https://openalex.org/I180923762', 'https://openalex.org/I4210126213', 'https://openalex.org/I4210141639', 'https://openalex.org/I44260953']}, {'id': 'https://openalex.org/I44260953', 'display_name': 'Heinrich Heine University Düsseldorf', 'ror': 'https://ror.org/024z2rq82', 'country_code': 'DE', 'type': 'education', 'lineage': ['https://openalex.org/I44260953']}], 'countries': ['DE'], 'is_corresponding': True, 'raw_author_name': 'Rüdiger Simon', 'raw_affiliation_strings': ['Institute for Developmental Genetics and Cluster of Excellence on Plant Sciences, Heinrich Heine University, Düsseldorf, Germany'], 'affiliations': [{'raw_affiliation_string': 'Institute for Developmental Genetics and Cluster of Excellence on Plant Sciences, Heinrich Heine University, Düsseldorf, Germany', 'institution_ids': ['https://openalex.org/I4210126213', 'https://openalex.org/I44260953']}]}], 'institution_assertions': [], 'countries_distinct_count': 2, 'institutions_distinct_count': 4, 'corresponding_author_ids': ['https://openalex.org/A5088362029', 'https://openalex.org/A5001481767'], 'corresponding_institution_ids': ['https://openalex.org/I4210126213', 'https://openalex.org/I44260953', 'https://openalex.org/I4210126213', 'https://openalex.org/I44260953'], 'apc_list': {'value': 1790, 'currency': 'GBP', 'value_usd': 2195, 'provenance': 'doaj'}, 'apc_paid': {'value': 1790, 'currency': 'GBP', 'value_usd': 2195, 'provenance': 'doaj'}, 'fwci': 1.546, 'has_fulltext': True, 'fulltext_origin': 'pdf', 'cited_by_count': 4, 'citation_normalized_percentile': {'value': 0.572124, 'is_in_top_1_percent': False, 'is_in_top_10_percent': False}, 'cited_by_percentile_year': {'min': 88, 'max': 90}, 'biblio': {'volume': '19', 'issue': '1', 'first_page': None, 'last_page': None}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T10184', 'display_name': 'Plant Molecular Biology Research', 'score': 0.9997, 'subfield': {'id': 'https://openalex.org/subfields/1110', 'display_name': 'Plant Science'}, 'field': {'id': 'https://openalex.org/fields/11', 'display_name': 'Agricultural and Biological Sciences'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, 'topics': [{'id': 'https://openalex.org/T10184', 'display_name': 'Plant Molecular Biology Research', 'score': 0.9997, 'subfield': {'id': 'https://openalex.org/subfields/1110', 'display_name': 'Plant Science'}, 'field': {'id': 'https://openalex.org/fields/11', 'display_name': 'Agricultural and Biological Sciences'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T11927', 'display_name': 'Plant Reproductive Biology', 'score': 0.9988, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T11237', 'display_name': 'Plant Gene Expression Analysis', 'score': 0.9978, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/bimolecular-fluorescence-complementation', 'display_name': 'Bimolecular fluorescence complementation', 'score': 0.8802646}, {'id': 'https://openalex.org/keywords/fluorescence-lifetime-imaging-microscopy', 'display_name': 'Fluorescence-lifetime imaging microscopy', 'score': 0.49403673}], 'concepts': [{'id': 'https://openalex.org/C96305047', 'wikidata': 'https://www.wikidata.org/wiki/Q367473', 'display_name': 'Förster resonance energy transfer', 'level': 3, 'score': 0.96428853}, {'id': 'https://openalex.org/C123243835', 'wikidata': 'https://www.wikidata.org/wiki/Q863465', 'display_name': 'Bimolecular fluorescence complementation', 'level': 3, 'score': 0.8802646}, {'id': 'https://openalex.org/C2780562374', 'wikidata': 'https://www.wikidata.org/wiki/Q3024956', 'display_name': 'Nicotiana benthamiana', 'level': 3, 'score': 0.757575}, {'id': 'https://openalex.org/C12554922', 'wikidata': 'https://www.wikidata.org/wiki/Q7100', 'display_name': 'Biophysics', 'level': 1, 'score': 0.5866436}, {'id': 'https://openalex.org/C11804247', 'wikidata': 'https://www.wikidata.org/wiki/Q896177', 'display_name': 'Protein–protein interaction', 'level': 2, 'score': 0.5639899}, {'id': 'https://openalex.org/C142613039', 'wikidata': 'https://www.wikidata.org/wiki/Q272983', 'display_name': 'Green fluorescent protein', 'level': 3, 'score': 0.51765233}, {'id': 'https://openalex.org/C57477423', 'wikidata': 'https://www.wikidata.org/wiki/Q1434196', 'display_name': 'Fluorescence-lifetime imaging microscopy', 'level': 3, 'score': 0.49403673}, {'id': 'https://openalex.org/C185592680', 'wikidata': 'https://www.wikidata.org/wiki/Q2329', 'display_name': 'Chemistry', 'level': 0, 'score': 0.48188668}, {'id': 'https://openalex.org/C2779491563', 'wikidata': 'https://www.wikidata.org/wiki/Q157892', 'display_name': 'Arabidopsis', 'level': 4, 'score': 0.4704363}, {'id': 'https://openalex.org/C144292202', 'wikidata': 'https://www.wikidata.org/wiki/Q898273', 'display_name': 'Protein domain', 'level': 3, 'score': 0.44880113}, {'id': 'https://openalex.org/C95444343', 'wikidata': 'https://www.wikidata.org/wiki/Q7141', 'display_name': 'Cell biology', 'level': 1, 'score': 0.35038167}, {'id': 'https://openalex.org/C91881484', 'wikidata': 'https://www.wikidata.org/wiki/Q191807', 'display_name': 'Fluorescence', 'level': 2, 'score': 0.33511156}, {'id': 'https://openalex.org/C70721500', 'wikidata': 'https://www.wikidata.org/wiki/Q177005', 'display_name': 'Computational biology', 'level': 1, 'score': 0.3227046}, {'id': 'https://openalex.org/C86803240', 'wikidata': 'https://www.wikidata.org/wiki/Q420', 'display_name': 'Biology', 'level': 0, 'score': 0.317776}, {'id': 'https://openalex.org/C55493867', 'wikidata': 'https://www.wikidata.org/wiki/Q7094', 'display_name': 'Biochemistry', 'level': 1, 'score': 0.25999367}, {'id': 'https://openalex.org/C143065580', 'wikidata': 'https://www.wikidata.org/wiki/Q3285695', 'display_name': 'Mutant', 'level': 3, 'score': 0.12786537}, {'id': 'https://openalex.org/C104317684', 'wikidata': 'https://www.wikidata.org/wiki/Q7187', 'display_name': 'Gene', 'level': 2, 'score': 0.11697939}, {'id': 'https://openalex.org/C121332964', 'wikidata': 'https://www.wikidata.org/wiki/Q413', 'display_name': 'Physics', 'level': 0, 'score': 0.10325572}, {'id': 'https://openalex.org/C62520636', 'wikidata': 'https://www.wikidata.org/wiki/Q944', 'display_name': 'Quantum mechanics', 'level': 1, 'score': 0.0}], 'mesh': [], 'locations_count': 3, 'locations': [{'is_oa': True, 'landing_page_url': 'https://doi.org/10.1186/s13007-023-01049-3', 'pdf_url': 'https://plantmethods.biomedcentral.com/counter/pdf/10.1186/s13007-023-01049-3', 'source': {'id': 'https://openalex.org/S184470774', 'display_name': 'Plant Methods', 'issn_l': '1746-4811', 'issn': ['1746-4811'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320256', 'host_organization_name': 'BioMed Central', 'host_organization_lineage': ['https://openalex.org/P4310319965', 'https://openalex.org/P4310320256'], 'host_organization_lineage_names': ['Springer Nature', 'BioMed Central'], 'type': 'journal'}, 'license': 'cc-by', 'license_id': 'https://openalex.org/licenses/cc-by', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, {'is_oa': True, 'landing_page_url': 'https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10375638', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S2764455111', 'display_name': 'PubMed Central', 'issn_l': None, 'issn': None, 'is_oa': True, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, {'is_oa': False, 'landing_page_url': 'https://pubmed.ncbi.nlm.nih.gov/37501124', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306525036', 'display_name': 'PubMed', 'issn_l': None, 'issn': None, 'is_oa': False, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.1186/s13007-023-01049-3', 'pdf_url': 'https://plantmethods.biomedcentral.com/counter/pdf/10.1186/s13007-023-01049-3', 'source': {'id': 'https://openalex.org/S184470774', 'display_name': 'Plant Methods', 'issn_l': '1746-4811', 'issn': ['1746-4811'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320256', 'host_organization_name': 'BioMed Central', 'host_organization_lineage': ['https://openalex.org/P4310319965', 'https://openalex.org/P4310320256'], 'host_organization_lineage_names': ['Springer Nature', 'BioMed Central'], 'type': 'journal'}, 'license': 'cc-by', 'license_id': 'https://openalex.org/licenses/cc-by', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'sustainable_development_goals': [{'display_name': 'Affordable and clean energy', 'score': 0.84, 'id': 'https://metadata.un.org/sdg/7'}], 'grants': [{'funder': 'https://openalex.org/F4320320879', 'funder_display_name': 'Deutsche Forschungsgemeinschaft', 'award_id': 'CRC1208'}, {'funder': 'https://openalex.org/F4320321951', 'funder_display_name': 'Heinrich-Heine-Universität Düsseldorf', 'award_id': None}], 'datasets': [], 'versions': [], 'referenced_works_count': 50, 'referenced_works': ['https://openalex.org/W1940712801', 'https://openalex.org/W1963834514', 'https://openalex.org/W1965169361', 'https://openalex.org/W1965330337', 'https://openalex.org/W1968176683', 'https://openalex.org/W1970635900', 'https://openalex.org/W1970962188', 'https://openalex.org/W1985241409', 'https://openalex.org/W1985991521', 'https://openalex.org/W1992385083', 'https://openalex.org/W1992646460', 'https://openalex.org/W1996765935', 'https://openalex.org/W2035136230', 'https://openalex.org/W2036023482', 'https://openalex.org/W2037611736', 'https://openalex.org/W2038902042', 'https://openalex.org/W2053266749', 'https://openalex.org/W2062893362', 'https://openalex.org/W2068805529', 'https://openalex.org/W2075018296', 'https://openalex.org/W2086762161', 'https://openalex.org/W2093931964', 'https://openalex.org/W2094517427', 'https://openalex.org/W2103750549', 'https://openalex.org/W2103942032', 'https://openalex.org/W2107021214', 'https://openalex.org/W2111518741', 'https://openalex.org/W2123210698', 'https://openalex.org/W2124471096', 'https://openalex.org/W2143310096', 'https://openalex.org/W2146189740', 'https://openalex.org/W2148387221', 'https://openalex.org/W2149748632', 'https://openalex.org/W2150892841', 'https://openalex.org/W2152553184', 'https://openalex.org/W2157498850', 'https://openalex.org/W2167064790', 'https://openalex.org/W2167279371', 'https://openalex.org/W2519934523', 'https://openalex.org/W2690619106', 'https://openalex.org/W2738496305', 'https://openalex.org/W2792179634', 'https://openalex.org/W2801102237', 'https://openalex.org/W2965752285', 'https://openalex.org/W2993152334', 'https://openalex.org/W2993530329', 'https://openalex.org/W3025520212', 'https://openalex.org/W3106659021', 'https://openalex.org/W4226028680', 'https://openalex.org/W4307406460'], 'related_works': ['https://openalex.org/W4385329934', 'https://openalex.org/W4311329190', 'https://openalex.org/W4200242823', 'https://openalex.org/W4200183111', 'https://openalex.org/W2902330969', 'https://openalex.org/W2408792444', 'https://openalex.org/W2054530395', 'https://openalex.org/W2034196816', 'https://openalex.org/W1992466727', 'https://openalex.org/W1975629253'], 'abstract_inverted_index': {'A': [0, 42], 'commonly': [1], 'used': [2, 62, 137, 283], 'approach': [3], 'to': [4, 32, 45, 63, 103, 145, 180, 219, 284], 'study': [5], 'the': [6, 25, 28, 114, 118, 122, 147, 168, 228, 295, 307, 312], 'interaction': [7, 275, 309], 'of': [8, 11, 27, 92, 117, 132, 152, 254, 294, 302], 'two': [9, 29, 33], 'proteins': [10, 35, 197, 256, 297], 'interest': [12], '(POIs)': [13], 'in': [14, 58, 78, 141, 158, 185, 187, 236, 245, 250, 266, 298, 311, 328], 'vivo': [15, 186, 237], 'is': [16, 48, 75, 315], 'measuring': [17], 'Förster': [18], 'Resonance': [19], 'Energy': [20], 'Transfer': [21], '(FRET).': [22], 'This': [23], 'requires': [24], 'expression': [26, 238, 319], 'POIs': [30], 'fused': [31], 'fluorescent': [34], 'that': [36, 195, 235, 260, 279], 'function': [37], 'as': [38, 222], 'a': [39, 85, 130, 223], 'FRET': [40, 47, 119, 123, 128, 178], 'pair.': [41], 'precise': [43], 'way': [44], 'record': [46], 'Fluorescence': [49], 'Lifetime': [50], 'IMaging': [51], '(FLIM)': [52], 'which': [53, 101], 'generates': [54], 'quantitative': [55], 'data': [56, 90], 'that,': [57], 'principle,': [59], 'can': [60, 281], 'be': [61, 282], 'resolve': [64], 'both': [65], 'complex': [66, 110, 252], 'structure': [67], 'and': [68, 109, 121, 149, 163, 171, 268, 292, 323, 333], 'protein': [69, 107, 174, 183, 262, 286, 304], 'affinities.': [70, 210], 'However,': [71], 'this': [72], 'potential': [73], 'resolution': [74], 'often': [76], 'lost': [77], 'many': [79], 'experimental': [80], 'approaches.': [81], 'Here': [82], 'we': [83, 136, 192, 216], 'introduce': [84], 'novel': [86], 'tool': [87, 170], 'for': [88, 134, 225], 'FLIM': [89, 213], 'analysis': [91, 99, 334], 'multiexponential': [93], 'decaying': [94], 'donor': [95], 'fluorophores,': [96], 'one': [97], 'pattern': [98], '(OPA),': [100], 'allows': [102], 'obtain': [104], 'information': [105, 289], 'about': [106, 290], 'affinity': [108], 'arrangement': [111], 'by': [112, 172], 'extracting': [113, 173], 'relative': [115], 'amplitude': [116], 'component': [120], 'transfer': [124], 'efficiency': [125], 'from': [126, 177, 239, 288], 'other': [127, 229], 'parameters.As': [129], 'proof': [131], 'concept': [133], 'OPA,': [135], 'FLIM-FRET,': [138], 'or': [139, 203], 'FLIM-FRET': [140], 'combination': [142], 'with': [143, 208, 214], 'BiFC': [144], 'reassess': [146], 'dimerization': [148], 'tetramerization': [150, 226], 'properties': [151], 'known': [153], 'interacting': [154, 296], 'MADS-domain': [155, 182, 196, 230, 255, 261], 'transcription': [156], 'factors': [157], 'Nicotiana': [159], 'benthamiana': [160, 190], 'leaf': [161], 'cells': [162], 'Arabidopsis': [164, 246], 'thaliana': [165], 'flowers.': [166], 'Using': [167], 'OPA': [169, 232, 280], 'BINDING': [175], 'efficiencies': [176], 'parameters': [179], 'dissect': [181], 'interactions': [184, 263, 305], 'transient': [188, 265], 'N.': [189], 'experiments,': [191], 'could': [193], 'show': [194], 'display': [198], 'similar': [199], 'proximities': [200], 'within': [201, 306], 'dimeric': [202], 'tetrameric': [204], 'complexes': [205], 'but': [206], 'bind': [207], 'variable': [209], 'By': [211], 'combining': [212], 'BiFC,': [215], 'were': [217], 'able': [218], 'identify': [220], 'SEPALLATA3': [221], 'mediator': [224], 'between': [227], 'factors.': [231], 'also': [233], 'revealed': [234], 'native': [240, 313], 'promoters': [241], 'at': [242], 'low': [243, 322], 'levels': [244, 320], 'flower': [247], 'meristems,': [248], 'makes': [249], 'situ': [251, 267], 'formation': [253], 'barely': [257], 'detectable.We': [258], 'conclude': [259, 278], 'are': [264, 321], 'may': [269], 'involve': [270], 'additional,': [271], 'so': [272], 'far': [273], 'unknown': [274], 'mediators.': [276], 'We': [277], 'separate': [285], 'binding': [287], 'proximity': [291], 'orientation': [293], 'their': [299], 'complexes.': [300], 'Visualization': [301], 'individual': [303], 'underlying': [308], 'networks': [310], 'environment': [314], 'still': [316], 'restrained': [317], 'if': [318], 'will': [324], 'require': [325], 'continuous': [326], 'improvements': [327], 'fluorophore': [329], 'labelling,': [330], 'instrumentation': [331], 'set-ups': [332], 'tools.': [335]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W4385329934', 'counts_by_year': [{'year': 2024, 'cited_by_count': 4}], 'updated_date': '2024-12-22T01:21:30.188324', 'created_date': '2023-07-29'}