Abstract: Diabetic foot infection (DFI) is one of the commonest diabetic complications, it's considered a major public health concern across the world, and its prevalence is rising, especially in developing countries. Globally, adults with diabetes accounted for 463 million in 2019 according to the International Diabetes Federation. Aim of this study was to identify the anaerobic and aerobic bacteria that causing and their therapeutic implications in Elnaw teaching hospital on diabetic clinics -Khartoum state. The study showed that there was no infection by anaerobic organism while the most frequently isolated was gram negative rods (70%)  and recorded (65%) on diabetic patients. S.aureus (30%) was the most isolated organism followed by E.coli (25%) in control group (non-diabetic), while the clinic room swabs revealed that Klebsiella. spp and S.aureus (20%) for each. Early diagnosis, optimal management and effective antibiotic therapy are necessary to prevent DFI and amputation.