Title: Immunoglobulins on the Surface of Lymphocytes
Abstract:Abstract The presence of immunoglobulin (Ig) on the surface of lymphocytes that were negative for Ig by immunofluorescence was investigated by combining a quantitative inhibition assay with immunofluo...Abstract The presence of immunoglobulin (Ig) on the surface of lymphocytes that were negative for Ig by immunofluorescence was investigated by combining a quantitative inhibition assay with immunofluorescence. Thymus cells; thymus, spleen and lymph node cells that had been passed through anti-Ig-coated plastic bead columns; and ϑ-positive mouse lymphomas were evaluated by using these two systems. These cell preparations had 0 to 1% of cells with detectable surface immunoglobulin by immunofluorescence. If the assumption is made that the fluorescent-positive cells were relatively homogeneous with respect to the amount of surface Ig, then it could be calculated that there was more Ig present in the various cell populations studied than was accountable by the small percentage of fluorescent-positive cells. Based on these calculations, the amount of Ig present in the different populations was found to be: fluorescent-positive cells: 5 to 14 ng N/106 cells; fluorescent-negative thymus cells: 0.01 ng N/106 cells; fluorescent-negative spleen and lymph node cells 0.07 to 0.1 ng N/106 cells. Three of four ϑ-positive lymphomas also had detectable Ig in amounts 3 to 10 times that found in normal thymus. Routinely there was more IgM found in both fluorescent-positive and -negative normal cells than IgG2.Read More