Title: Buoyant density of hepatitis C virus recovered from infected hosts: Two different features in sucrose equilibrium density-gradient centrifugation related to degree of liver inflammation
Abstract: HepatologyVolume 19, Issue 2 p. 296-302 Original ArticleFree Access Buoyant density of hepatitis C virus recovered from infected hosts: Two different features in sucrose equilibrium density-gradient centrifugation related to degree of liver inflammation Tatsuya Kanto, Tatsuya Kanto First Department of Medicine, Osaka University Medical School, Osaka 565, JapanSearch for more papers by this authorNorio Hayashi M.D., Corresponding Author Norio Hayashi M.D. First Department of Medicine, Osaka University Medical School, Osaka 565, JapanFirst Department of Medicine, Osaka University Medical School, 2-2 Yamada-oka, Suita, Osaka 565, Japan===Search for more papers by this authorTetsuo Takehara, Tetsuo Takehara First Department of Medicine, Osaka University Medical School, Osaka 565, JapanSearch for more papers by this authorHideki Hagiwara, Hideki Hagiwara First Department of Medicine, Osaka University Medical School, Osaka 565, JapanSearch for more papers by this authorEiji Mita, Eiji Mita First Department of Medicine, Osaka University Medical School, Osaka 565, JapanSearch for more papers by this authorMasafumi Naito, Masafumi Naito First Department of Medicine, Osaka University Medical School, Osaka 565, JapanSearch for more papers by this authorAkinori Kasahara, Akinori Kasahara First Department of Medicine, Osaka University Medical School, Osaka 565, JapanSearch for more papers by this authorHideyuki Fusamoto, Hideyuki Fusamoto First Department of Medicine, Osaka University Medical School, Osaka 565, JapanSearch for more papers by this authorTakenobu Kamada, Takenobu Kamada First Department of Medicine, Osaka University Medical School, Osaka 565, JapanSearch for more papers by this author Tatsuya Kanto, Tatsuya Kanto First Department of Medicine, Osaka University Medical School, Osaka 565, JapanSearch for more papers by this authorNorio Hayashi M.D., Corresponding Author Norio Hayashi M.D. First Department of Medicine, Osaka University Medical School, Osaka 565, JapanFirst Department of Medicine, Osaka University Medical School, 2-2 Yamada-oka, Suita, Osaka 565, Japan===Search for more papers by this authorTetsuo Takehara, Tetsuo Takehara First Department of Medicine, Osaka University Medical School, Osaka 565, JapanSearch for more papers by this authorHideki Hagiwara, Hideki Hagiwara First Department of Medicine, Osaka University Medical School, Osaka 565, JapanSearch for more papers by this authorEiji Mita, Eiji Mita First Department of Medicine, Osaka University Medical School, Osaka 565, JapanSearch for more papers by this authorMasafumi Naito, Masafumi Naito First Department of Medicine, Osaka University Medical School, Osaka 565, JapanSearch for more papers by this authorAkinori Kasahara, Akinori Kasahara First Department of Medicine, Osaka University Medical School, Osaka 565, JapanSearch for more papers by this authorHideyuki Fusamoto, Hideyuki Fusamoto First Department of Medicine, Osaka University Medical School, Osaka 565, JapanSearch for more papers by this authorTakenobu Kamada, Takenobu Kamada First Department of Medicine, Osaka University Medical School, Osaka 565, JapanSearch for more papers by this author First published: February 1994 https://doi.org/10.1002/hep.1840190206Citations: 84AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract Hepatitis C virus is reported to have a low buoyant density in sucrose. To determine the density of hepatitis C virus in the circulation of infected hosts and its association with the degree of liver inflammation, we examined serum samples from 10 patients who were positive for both hepatitis C virus antibody (C100 antigen) antibody and serum hepatitis C virus RNA. After the serum was ultracentrifuged in sucrose density gradient (10 to 60), the hepatitis C virus RNA titer in each collected fraction was quantified by means of competitive reverse transcription–polymerase chain reaction. In samples from five blood donors, the hepatitis C virus RNA titer had a single peak at fractions with densities of 1.08 to 1.11 gm/ml. In samples from five patients with ALT abnormalities, the titer had two peaks at fractions with 1.09 to 1.10 gm/ml and 1.22 to 1.25 gm/ml. After the selected samples were treated with detergents and ultracentrifuged, the titer in the 1.08 to 1.11 gm/ml fractions decreased and that in the 1.22 to 1.25 gm fractions increased. This result implied that the hepatitis C virus density changed with removal of the viral envelope by lipid solvents. Thus the buoyant density of hepatitis C virus in sucrose was 1.08 to 1.11 gm/ml for an intact virion and 1.22 to 1.25 gm/ml for what was presumed to be a nucleocapsid. These results demonstrated that HCV virion is a dominant form in the circulation of blood donors without ALT abnormalities. In patients with liver inflammation HCV particles with higher densities of 1.22 to 1.25 gm/ml coexist with virion in the circulation, which might be presumed nucleocapsids. (Hepatology 1994;19:296–302). Citing Literature Volume19, Issue2February 1994Pages 296-302 ReferencesRelatedInformation
Publication Year: 1994
Publication Date: 1994-02-01
Language: en
Type: article
Indexed In: ['crossref']
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Cited By Count: 84
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