Title: Wnt inhibitory factor 1 ameliorated diabetic retinopathy through the <scp>AMPK/mTOR</scp> pathway‐mediated mitochondrial function
Abstract: The FASEB JournalVolume 36, Issue 10 e22531 RESEARCH ARTICLE Wnt inhibitory factor 1 ameliorated diabetic retinopathy through the AMPK/mTOR pathway-mediated mitochondrial function Jing Zou, Jing Zou Eye Center of Xiangya Hospital, Central South University, Changsha, P.R. China National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, P.R. ChinaSearch for more papers by this authorWei Tan, Wei Tan Eye Center of Xiangya Hospital, Central South University, Changsha, P.R. China National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, P.R. ChinaSearch for more papers by this authorKangcheng Liu, Kangcheng Liu Eye Center of Xiangya Hospital, Central South University, Changsha, P.R. China National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, P.R. ChinaSearch for more papers by this authorBolin Chen, Bolin Chen Eye Center of Xiangya Hospital, Central South University, Changsha, P.R. China National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, P.R. ChinaSearch for more papers by this authorTianQi Duan, TianQi Duan Eye Center of Xiangya Hospital, Central South University, Changsha, P.R. China National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, P.R. ChinaSearch for more papers by this authorHuizhuo Xu, Corresponding Author Huizhuo Xu [email protected] orcid.org/0000-0002-5715-9384 Eye Center of Xiangya Hospital, Central South University, Changsha, P.R. China National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, P.R. China Correspondence Huizhuo Xu, Eye Center of Xiangya Hospital, Central South University, No. 87, Xiangya Road, Kaifu District, Changsha 410008, Hunan Province, P.R. China. Email: [email protected] for more papers by this author Jing Zou, Jing Zou Eye Center of Xiangya Hospital, Central South University, Changsha, P.R. China National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, P.R. ChinaSearch for more papers by this authorWei Tan, Wei Tan Eye Center of Xiangya Hospital, Central South University, Changsha, P.R. China National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, P.R. ChinaSearch for more papers by this authorKangcheng Liu, Kangcheng Liu Eye Center of Xiangya Hospital, Central South University, Changsha, P.R. China National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, P.R. ChinaSearch for more papers by this authorBolin Chen, Bolin Chen Eye Center of Xiangya Hospital, Central South University, Changsha, P.R. China National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, P.R. ChinaSearch for more papers by this authorTianQi Duan, TianQi Duan Eye Center of Xiangya Hospital, Central South University, Changsha, P.R. China National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, P.R. ChinaSearch for more papers by this authorHuizhuo Xu, Corresponding Author Huizhuo Xu [email protected] orcid.org/0000-0002-5715-9384 Eye Center of Xiangya Hospital, Central South University, Changsha, P.R. China National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, P.R. China Correspondence Huizhuo Xu, Eye Center of Xiangya Hospital, Central South University, No. 87, Xiangya Road, Kaifu District, Changsha 410008, Hunan Province, P.R. China. Email: [email protected] for more papers by this author First published: 05 September 2022 https://doi.org/10.1096/fj.202200366RRRead the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract Diabetic retinopathy (DR) is one of the most common complications of diabetes mellitus and will lead to visual impairment. We aim to explore the effects and mechanisms of wnt inhibitory factor 1 (WIF1) in the progression of DR. To establish DR in vitro and in vivo, human retinal pigment epithelium (RPE) cell line ARPE-19 was treated with high-glucose (HG) and diabetic mice models were induced by streptozotocin (STZ), respectively. Different dose of recombinant WIF1 protein was used to treat DR. qRT-PCR and western blotting results demonstrated that WIF1 was downregulated, while VEGFA was upregulated in HG-induced ARPE-19 cells. WIF1 overexpression promoted cell migration. The ARPE-19 cells culture medium treated with WIF1 inhibited retinal endothelial cell tube formation and downregulated VEGFA expression. Moreover, WIF1 decreased the levels of ROS and MDA, while increasing the activity of SOD and GPX. WIF1 increased the ΔΨm in the mitochondria and downregulated the expression of mitochondrial autophagy-related proteins including Parkin, Pink1, LC3-II/LC3-I ratio, cleaved caspase 3, and cyt-c, which ameliorated mitochondrial dysfunction. The in vivo studies further demonstrated the consistent effects of WIF1 in STZ-induced mice. Taken together, WIF1 ameliorated mitochondrial dysfunction in DR by downregulating the AMPK/mTOR pathway. Open Research DATA AVAILABILITY STATEMENT All data generated or analyzed during this study are included in this published article. Supporting Information Filename Description fsb222531-sup-0001-FigureS1.tifimage/tif, 740.8 KB Figure S1 fsb222531-sup-0002-FigureS1caption.docxWord 2007 document , 14.5 KB Figure S1caption Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume36, Issue10October 2022e22531 RelatedInformation
Publication Year: 2022
Publication Date: 2022-09-05
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 12
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