Title: Immunoelectron Microscopy: High Resolution Immunocytochemistry
Abstract: Subcellular structures as seen in the electron microscope require correlation to a biological function to increase our knowledge about fundamental cellular processes. Immunoelectron microscopy is a tool to map macromolecules to these structures with high spatial resolution within the context of the cell. Different methods of sample preparation and immunolabeling strategies are available and must be selected according to the question under investigation. Sample preparation requires immobilization/fixation. This can be achieved chemically followed by conventional or PLT embedding or Tokuyasu cryosectioning. Alternatively, cryo-preparation methods (high-pressure-freezing followed by freeze-substitution and resin embedding at low temperature) can be applied. Basic immunolabeling strategies are pre-embedding labeling of permeabilized chemically fixed samples or on-section labeling which is compatible with different sample preparation methods. Immunolabeling without thin sectioning is performed in whole mount labeling and freeze-fracture cytochemistry. Immunogold labeling allows quantification by stereological methods and statistical evaluation.
Publication Year: 2023
Publication Date: 2023-01-01
Language: en
Type: book-chapter
Indexed In: ['crossref']
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