Title: Erythropoietin production in hepatocellular carcinoma cells associated with polycythemia: Immunohistochemical evidence
Abstract: HepatologyVolume 18, Issue 6 p. 1357-1362 Other Clinical StudyFree Access Erythropoietin production in hepatocellular carcinoma cells associated with polycythemia: Immunohistochemical evidence Shotaro Sakisaka M. D. Ph.D., Corresponding Author Shotaro Sakisaka M. D. Ph.D. Second Department of Medicine, Kurume University School of Medicine, Kurume 830, JapanSecond Department of Medicine, Kurume University School of Medicine, 67 Asahi-machi, Kurume 830, Japan===Search for more papers by this authorMasahide Watanabe, Masahide Watanabe Second Department of Medicine, Kurume University School of Medicine, Kurume 830, JapanSearch for more papers by this authorHideo Tateishi, Hideo Tateishi Second Department of Medicine, Kurume University School of Medicine, Kurume 830, JapanSearch for more papers by this authorMasaru Harada, Masaru Harada Second Department of Medicine, Kurume University School of Medicine, Kurume 830, JapanSearch for more papers by this authorSatoshi Shakado, Satoshi Shakado Second Department of Medicine, Kurume University School of Medicine, Kurume 830, JapanSearch for more papers by this authorYoshihiro Mimura, Yoshihiro Mimura Second Department of Medicine, Kurume University School of Medicine, Kurume 830, JapanSearch for more papers by this authorKazuhisa Gondo, Kazuhisa Gondo Second Department of Medicine, Kurume University School of Medicine, Kurume 830, JapanSearch for more papers by this authorMasao Yoshitake, Masao Yoshitake Second Department of Medicine, Kurume University School of Medicine, Kurume 830, JapanSearch for more papers by this authorKazunori Noguchi, Kazunori Noguchi Second Department of Medicine, Kurume University School of Medicine, Kurume 830, JapanSearch for more papers by this authorTeruko Hino, Teruko Hino Second Department of Medicine, Kurume University School of Medicine, Kurume 830, JapanSearch for more papers by this authorRyuichi Nohno, Ryuichi Nohno Second Department of Medicine, Kurume University School of Medicine, Kurume 830, JapanSearch for more papers by this authorYasuo Majima, Yasuo Majima Second Department of Medicine, Kurume University School of Medicine, Kurume 830, JapanSearch for more papers by this authorKenji Hirai, Kenji Hirai Second Department of Medicine, Kurume University School of Medicine, Kurume 830, JapanSearch for more papers by this authorMichio Sata, Michio Sata Second Department of Medicine, Kurume University School of Medicine, Kurume 830, JapanSearch for more papers by this authorHiroshi Yoshida, Hiroshi Yoshida Second Department of Medicine, Kurume University School of Medicine, Kurume 830, JapanSearch for more papers by this authorKyuichi Tanikawa, Kyuichi Tanikawa Second Department of Medicine, Kurume University School of Medicine, Kurume 830, JapanSearch for more papers by this author Shotaro Sakisaka M. D. Ph.D., Corresponding Author Shotaro Sakisaka M. D. Ph.D. Second Department of Medicine, Kurume University School of Medicine, Kurume 830, JapanSecond Department of Medicine, Kurume University School of Medicine, 67 Asahi-machi, Kurume 830, Japan===Search for more papers by this authorMasahide Watanabe, Masahide Watanabe Second Department of Medicine, Kurume University School of Medicine, Kurume 830, JapanSearch for more papers by this authorHideo Tateishi, Hideo Tateishi Second Department of Medicine, Kurume University School of Medicine, Kurume 830, JapanSearch for more papers by this authorMasaru Harada, Masaru Harada Second Department of Medicine, Kurume University School of Medicine, Kurume 830, JapanSearch for more papers by this authorSatoshi Shakado, Satoshi Shakado Second Department of Medicine, Kurume University School of Medicine, Kurume 830, JapanSearch for more papers by this authorYoshihiro Mimura, Yoshihiro Mimura Second Department of Medicine, Kurume University School of Medicine, Kurume 830, JapanSearch for more papers by this authorKazuhisa Gondo, Kazuhisa Gondo Second Department of Medicine, Kurume University School of Medicine, Kurume 830, JapanSearch for more papers by this authorMasao Yoshitake, Masao Yoshitake Second Department of Medicine, Kurume University School of Medicine, Kurume 830, JapanSearch for more papers by this authorKazunori Noguchi, Kazunori Noguchi Second Department of Medicine, Kurume University School of Medicine, Kurume 830, JapanSearch for more papers by this authorTeruko Hino, Teruko Hino Second Department of Medicine, Kurume University School of Medicine, Kurume 830, JapanSearch for more papers by this authorRyuichi Nohno, Ryuichi Nohno Second Department of Medicine, Kurume University School of Medicine, Kurume 830, JapanSearch for more papers by this authorYasuo Majima, Yasuo Majima Second Department of Medicine, Kurume University School of Medicine, Kurume 830, JapanSearch for more papers by this authorKenji Hirai, Kenji Hirai Second Department of Medicine, Kurume University School of Medicine, Kurume 830, JapanSearch for more papers by this authorMichio Sata, Michio Sata Second Department of Medicine, Kurume University School of Medicine, Kurume 830, JapanSearch for more papers by this authorHiroshi Yoshida, Hiroshi Yoshida Second Department of Medicine, Kurume University School of Medicine, Kurume 830, JapanSearch for more papers by this authorKyuichi Tanikawa, Kyuichi Tanikawa Second Department of Medicine, Kurume University School of Medicine, Kurume 830, JapanSearch for more papers by this author First published: December 1993 https://doi.org/10.1002/hep.1840180612Citations: 34AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract Patients with hepatocellular carcinoma sometimes have erythrocytosis and high plasma erythropoietin levels. However, previous studies have not revealed direct evidence that the carcinoma cells produce the erythropoietin. To address this question, we carried out light and electron microscopic immunohistochemical studies, using a human erythropoietin antibody to the liver in three male patients with hepatocellular carcinoma and erythrocytosis. α-Fetoprotein localization was also examined in serial liver sections by light microscopic immunohistochemistry with an antibody to α-fetoprotein. All three patients demonstrated high hemoglobin levels (16.7, 17.6 and 18.1 gm/dl) and high plasma erythropoietin levels (227, 266 and 280 mU/ml). In one patient the plasma erythropoietin level in the hepatic vein was significantly higher than that in the hepatic artery. The levels of plasma erythropoietin, as well as such tumor markers for hepatocellular carcinoma as serum α-fetoprotein and plasma des-γ-carboxyprothrombin, were significantly reduced after treatment with an anticancer drug, cisplatin. Light microscopic immunohistochemistry showed that erythropoietin was definitely present in the cytoplasm of the hepatocellular carcinoma cells, but not in normal hepatocytes around the carcinoma lesion or in other nonparenchymal cells such as vascular endothelial cells and Kupffer cells. In electron microscopic immunohistochemistry, reaction products for erythropoietin were revealed in the cisternae of the endoplasmic reticulum in the carcinoma cells, suggesting the production of erythropoietin by these cells. Light microscopic immunohistochemistry showed that α-fetoprotein was localized in the hepatocellular carcinoma cells that were erythropoietin positive in the serial sections. These findings indicated that hepatocellular carcinoma cells produced erythropoietin as well as α-fetoprotein in these cases, leading to the complication of erythrocytosis. (HEPATOLOGY 1993;18:1357–1362.) Citing Literature Volume18, Issue6December 1993Pages 1357-1362 ReferencesRelatedInformation
Publication Year: 1993
Publication Date: 1993-12-01
Language: en
Type: article
Indexed In: ['crossref']
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Cited By Count: 8
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