Title: Identification of the tryptophan residue located at the saccharide binding site of castor bean hemagglutinin.
Abstract: The tryptophan residue present at the saccharide-binding site of castor bean hemagglutinin (CBH) was identified. A peptide containing a modified tryptophan residue was isolated from the tryptic digest of S-carboxymethylated B-chain obtained from an inactive derivative of CBH (2-Oxa-CBH), in which two tryptophan residues/mol were oxidized with N-bromosuccinimide, by gel filtration on a Sephadex G-50 followed by high performance liquid chromatography. Analytical data for the isolated peptide indicated that the tryptophan residue at position 131 on the B-chain was modified in 2-Oxa-CBH. From these and earlier results, it is suggested that the tryptophan residue at 131 on each B-chain is closely associated with the saccharide-binding activity of CBH. The specific role of tryptophan residue at 131 in the saccharide-binding site of CBH is also discussed.