Title: Determination of Genetic Diversity in Tall Fescue with AFLP Markers
Abstract: Crop ScienceVolume 42, Issue 3 p. 944-950 Plant genetic resources Determination of Genetic Diversity in Tall Fescue with AFLP Markers M. A. Rouf Mian, Corresponding Author M. A. Rouf Mian [email protected] Forage Biotechnology Group, The Samuel Roberts Noble Foundation, Ardmore, OK, 73401Corresponding author ([email protected])Search for more papers by this authorAndrew A. Hopkins, Andrew A. Hopkins Forage Biotechnology Group, The Samuel Roberts Noble Foundation, Ardmore, OK, 73401Search for more papers by this authorJohn C. Zwonitzer, John C. Zwonitzer Forage Biotechnology Group, The Samuel Roberts Noble Foundation, Ardmore, OK, 73401Search for more papers by this author M. A. Rouf Mian, Corresponding Author M. A. Rouf Mian [email protected] Forage Biotechnology Group, The Samuel Roberts Noble Foundation, Ardmore, OK, 73401Corresponding author ([email protected])Search for more papers by this authorAndrew A. Hopkins, Andrew A. Hopkins Forage Biotechnology Group, The Samuel Roberts Noble Foundation, Ardmore, OK, 73401Search for more papers by this authorJohn C. Zwonitzer, John C. Zwonitzer Forage Biotechnology Group, The Samuel Roberts Noble Foundation, Ardmore, OK, 73401Search for more papers by this author First published: 01 May 2002 https://doi.org/10.2135/cropsci2002.9440Citations: 28Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Abstract Little information is available regarding genetic variation in tall fescue (Festuca arundinacea Schreb). Such information is important in constructing mapping populations and targeting germplasm collection and utilization. The objective of this study was to evaluate the genetic diversity among a number of tall fescue accessions, cultivars, and selected genotypes. In one experiment, 16 persistent tall fescue plants (genotypes) were assayed by a fluorescence-labeled amplified fragment length polymorphism (AFLP) detection method using DNA samples from individual plants. In a second experiment, AFLP diversity among 18 tall fescue populations, including sources of ‘KY-31’, cultivars derived from KY-31 and accessions collected from Oklahoma and Texas, was assessed by an infrared dye-labeling method using DNA samples bulked by each population. On the basis of 461 AFLP markers from six primer combinations, the 16 individual plants were clustered in groups that largely supported the known origins of these plants. On the basis of genetic divergence, several candidate parents were identified for constructing a mapping population. Analysis of bulked DNA samples revealed that sources of KY-31 and cultivars derived from KY-31 generally clustered separately from accessions collected in the southern Great Plains. Such accessions thus provide an additional source of genetic variation for tall fescue breeding programs. Citing Literature Volume42, Issue3May–June 2002Pages 944-950 RelatedInformation
Publication Year: 2002
Publication Date: 2002-01-01
Language: en
Type: article
Indexed In: ['crossref']
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Cited By Count: 22
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