Title: Multiple cross-reactive self-ligands for Borrelia burgdorferi-specific HLA-DR4-restricted T cells
Abstract: European Journal of ImmunologyVolume 30, Issue 2 p. 448-457 ArticleFree Access Multiple cross-reactive self-ligands for Borrelia burgdorferi-specific HLA-DR4-restricted T cells Bert Maier, Bert Maier Deutsches Rheumaforschungszentrum Berlin, GermanySearch for more papers by this authorMarc Molinger, Marc Molinger Deutsches Rheumaforschungszentrum Berlin, GermanySearch for more papers by this authorAndrew P. Cope, Andrew P. Cope Department of Medicine, Stanford University, School of Medicine, Stanford, USASearch for more papers by this authorLars Fugger, Lars Fugger Department of Medicine, Stanford University, School of Medicine, Stanford, USASearch for more papers by this authorJens Schneider-Mergener, Jens Schneider-Mergener Institut für Medizinische Immunologie, Universitätsklinikum Charité, Humboldt-Universität zu Berlin, Berlin, GermanySearch for more papers by this authorGrete Sønderstrup, Grete Sønderstrup Department of Medicine, Stanford University, School of Medicine, Stanford, USASearch for more papers by this authorThomas Kamradt, Corresponding Author Thomas Kamradt [email protected] Deutsches Rheumaforschungszentrum Berlin, GermanyDRFZ, Monbijoustr. 2, D-10117 Berlin, Germany Fax: + 49-30-28460-764Search for more papers by this authorAchim Kramer, Achim Kramer Institut für Medizinische Immunologie, Universitätsklinikum Charité, Humboldt-Universität zu Berlin, Berlin, GermanySearch for more papers by this author Bert Maier, Bert Maier Deutsches Rheumaforschungszentrum Berlin, GermanySearch for more papers by this authorMarc Molinger, Marc Molinger Deutsches Rheumaforschungszentrum Berlin, GermanySearch for more papers by this authorAndrew P. Cope, Andrew P. Cope Department of Medicine, Stanford University, School of Medicine, Stanford, USASearch for more papers by this authorLars Fugger, Lars Fugger Department of Medicine, Stanford University, School of Medicine, Stanford, USASearch for more papers by this authorJens Schneider-Mergener, Jens Schneider-Mergener Institut für Medizinische Immunologie, Universitätsklinikum Charité, Humboldt-Universität zu Berlin, Berlin, GermanySearch for more papers by this authorGrete Sønderstrup, Grete Sønderstrup Department of Medicine, Stanford University, School of Medicine, Stanford, USASearch for more papers by this authorThomas Kamradt, Corresponding Author Thomas Kamradt [email protected] Deutsches Rheumaforschungszentrum Berlin, GermanyDRFZ, Monbijoustr. 2, D-10117 Berlin, Germany Fax: + 49-30-28460-764Search for more papers by this authorAchim Kramer, Achim Kramer Institut für Medizinische Immunologie, Universitätsklinikum Charité, Humboldt-Universität zu Berlin, Berlin, GermanySearch for more papers by this author First published: 31 August 2000 https://doi.org/10.1002/1521-4141(200002)30:2<448::AID-IMMU448>3.0.CO;2-9Citations: 31AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract T cell recognition of self antigens is a key event in the pathogenesis of autoimmune diseases. To date, the initial events that trigger autoreactive T cells are unknown. The "molecular mimicry" hypothesis predicts that during an infection T cells that recognize both a microbial antigen and a related self peptide become activated and cause autoimmune disease. We have systematically examined the recognition of self antigens by HLA-DR4-restricted T cells specific for peptides of the outer surface protein A (OspA) of Borrelia burgdorferi, the etiological agent of Lyme disease. We used the peptide spot synthesis technique for complete peptide substitution analyses of two immunodominant OspA epitopes. Each amino acid residue of the epitopes was substituted with all 20 naturally occurring amino acids and the altered peptides were tested for recognition by a panel of OspA-specific T cells. The binding motifs (supertopes) revealed by these analyses were used to screen public databases for matching human or murine peptides. Several hundred peptides were identified by this search and synthesized. Of these, 28 were recognized by OspA-specific T cells. Thus, T cell cross-reactivity is a common phenomenon and the existence of cross-reactive epitopes alone does not imply molecular mimicry-mediated pathology and autoimmunity. Citing Literature Volume30, Issue2February 2000Pages 448-457 RelatedInformation