Abstract:Report of the Activity of iGEM HokkaidoUCircularization of a protein is one of the ways to enhance its stability against various temperatures and pH levels.We challenged ourselves to circularize prote...Report of the Activity of iGEM HokkaidoUCircularization of a protein is one of the ways to enhance its stability against various temperatures and pH levels.We challenged ourselves to circularize proteins using self-assembling peptides and linkers.Self-assembling peptide (SAP) is an amphiphilic peptide which self-assembles under physicochemical conditions.We made SAPs fused to link ends of the protein.We also inserted cysteine residues in the linker which is essential for the circularization so that they form a disulfide bond.This linker is expected to be applicable to most proteins because it automatically adjusts its length.These two elements -the self-assembling peptide and the linker -enable the protein to be circularized.Read More