Title: Localisation of Amylose and Amylopectin in Starch Granules Using Enzyme-Gold Labelling
Abstract: Starch - StärkeVolume 51, Issue 5 p. 163-172 Research Paper Localisation of Amylose and Amylopectin in Starch Granules Using Enzyme-Gold Labelling Neil J. Atkin, Neil J. Atkin Institute for Applied Biology, Department of Biology, The University of York, P.O. Box 373, Heslington, York YO1O 5YW, United KingdomSearch for more papers by this authorSophia L. Cheng, Sophia L. Cheng Institute for Applied Biology, Department of Biology, The University of York, P.O. Box 373, Heslington, York YO1O 5YW, United KingdomSearch for more papers by this authorRukmal M. Abeysekera, Rukmal M. AbeysekeraSearch for more papers by this authorAnthony W. Robards, Anthony W. Robards Institute for Applied Biology, Department of Biology, The University of York, P.O. Box 373, Heslington, York YO1O 5YW, United KingdomSearch for more papers by this author Neil J. Atkin, Neil J. Atkin Institute for Applied Biology, Department of Biology, The University of York, P.O. Box 373, Heslington, York YO1O 5YW, United KingdomSearch for more papers by this authorSophia L. Cheng, Sophia L. Cheng Institute for Applied Biology, Department of Biology, The University of York, P.O. Box 373, Heslington, York YO1O 5YW, United KingdomSearch for more papers by this authorRukmal M. Abeysekera, Rukmal M. AbeysekeraSearch for more papers by this authorAnthony W. Robards, Anthony W. Robards Institute for Applied Biology, Department of Biology, The University of York, P.O. Box 373, Heslington, York YO1O 5YW, United KingdomSearch for more papers by this author First published: 05 July 1999 https://doi.org/10.1002/(SICI)1521-379X(199905)51:5<163::AID-STAR163>3.0.CO;2-FCitations: 41AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Abstract The independent localisation of amylose and amylopectin in a range of dry and hydrated native starch granules with varying amylose content (0—70 %) has been indirectly visualised using enzyme-gold cytochemical markers. Increasing amylose content was clearly demonstrated to result in distinct changes in granule architecture. In the absence of amylose (waxy maize starch) a framework of closely packed concentric layers of amylopectin exists in the granules. Low amylose content (potato starch) results in alternating layers of densely packed amylopectin and amylose molecules. High amylose content (amylomaize starch) granules were shown to possess an amylopectin centre surrounded by an amylose periphery encapsulated by an amylopectin surface. Elongated granules without the amylopectin centre were also observed in high amylose starches suggesting a relationship between amylopectin, amylose and granule shape. A model of starch granule architecture is proposed where increased compartmentalisation of amylose and amylopectin is observed in granules containing increasing levels of amylose. Citing Literature Volume51, Issue5May 1999Pages 163-172 RelatedInformation
Publication Year: 1999
Publication Date: 1999-05-01
Language: en
Type: article
Indexed In: ['crossref']
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Cited By Count: 2
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