Abstract: Standard operating procedure for using the gassing manifold The gassing manifold is used for replacing the headspace of vials with N2 (or N2:CO2) or for degassing solutions. It is useful for preparing anoxic incubations, media and buffers before bringing them in closed vials into the anoxic chamber. The manifold is equipped with an electronic controller that automatically switches between the gas inlet and the vacuum pump and performs multiple cycles of headspace flushing.