Publication Information

{'id': 'https://openalex.org/W4246058695', 'doi': 'https://doi.org/10.1128/mcb.13.4.2441-2455.1993', 'title': 'Structural Alterations of the Nucleolus in Mutants of <i>Saccharomyces cerevisiae</i> Defective in RNA Polymerase I', 'display_name': 'Structural Alterations of the Nucleolus in Mutants of <i>Saccharomyces cerevisiae</i> Defective in RNA Polymerase I', 'publication_year': 1993, 'publication_date': '1993-04-01', 'ids': {'openalex': 'https://openalex.org/W4246058695', 'doi': 'https://doi.org/10.1128/mcb.13.4.2441-2455.1993', 'pmid': 'https://pubmed.ncbi.nlm.nih.gov/8455621'}, 'language': 'en', 'primary_location': {'is_oa': False, 'landing_page_url': 'https://doi.org/10.1128/mcb.13.4.2441-2455.1993', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S81455967', 'display_name': 'Molecular and Cellular Biology', 'issn_l': '0270-7306', 'issn': ['0270-7306', '1067-8824', '1098-5549'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320547', 'host_organization_name': 'Taylor & Francis', 'host_organization_lineage': ['https://openalex.org/P4310320547'], 'host_organization_lineage_names': ['Taylor & Francis'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': ['crossref', 'pubmed'], 'open_access': {'is_oa': False, 'oa_status': 'closed', 'oa_url': None, 'any_repository_has_fulltext': False}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5082817485', 'display_name': 'Melanie L. Oakes', 'orcid': 'https://orcid.org/0009-0002-2369-4810'}, 'institutions': [{'id': 'https://openalex.org/I204250578', 'display_name': 'University of California, Irvine', 'ror': 'https://ror.org/04gyf1771', 'country_code': 'US', 'type': 'education', 'lineage': ['https://openalex.org/I204250578']}], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Melanie Oakes', 'raw_affiliation_strings': ['Department of Biological Chemistry, University of California, Irvine, California 92717-1700'], 'affiliations': [{'raw_affiliation_string': 'Department of Biological Chemistry, University of California, Irvine, California 92717-1700', 'institution_ids': ['https://openalex.org/I204250578']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5051628580', 'display_name': 'Yasuhisa Nogi', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I204250578', 'display_name': 'University of California, Irvine', 'ror': 'https://ror.org/04gyf1771', 'country_code': 'US', 'type': 'education', 'lineage': ['https://openalex.org/I204250578']}], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Yasuhisa Nogi', 'raw_affiliation_strings': ['Department of Biological Chemistry, University of California, Irvine, California 92717-1700'], 'affiliations': [{'raw_affiliation_string': 'Department of Biological Chemistry, University of California, Irvine, California 92717-1700', 'institution_ids': ['https://openalex.org/I204250578']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5110317597', 'display_name': 'Michael W. Clark', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I204250578', 'display_name': 'University of California, Irvine', 'ror': 'https://ror.org/04gyf1771', 'country_code': 'US', 'type': 'education', 'lineage': ['https://openalex.org/I204250578']}], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Michael W. Clark', 'raw_affiliation_strings': ['Department of Biological Chemistry, University of California, Irvine 92717-1700.'], 'affiliations': [{'raw_affiliation_string': 'Department of Biological Chemistry, University of California, Irvine 92717-1700.', 'institution_ids': ['https://openalex.org/I204250578']}]}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5072834548', 'display_name': 'Michio Nomura', 'orcid': 'https://orcid.org/0000-0003-1043-2811'}, 'institutions': [{'id': 'https://openalex.org/I204250578', 'display_name': 'University of California, Irvine', 'ror': 'https://ror.org/04gyf1771', 'country_code': 'US', 'type': 'education', 'lineage': ['https://openalex.org/I204250578']}], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Masayasu Nomura', 'raw_affiliation_strings': ['Department of Biological Chemistry, University of California, Irvine, California 92717-1700'], 'affiliations': [{'raw_affiliation_string': 'Department of Biological Chemistry, University of California, Irvine, California 92717-1700', 'institution_ids': ['https://openalex.org/I204250578']}]}], 'institution_assertions': [], 'countries_distinct_count': 1, 'institutions_distinct_count': 1, 'corresponding_author_ids': [], 'corresponding_institution_ids': [], 'apc_list': None, 'apc_paid': None, 'fwci': 1.062, 'has_fulltext': False, 'cited_by_count': 40, 'citation_normalized_percentile': {'value': 0.789646, 'is_in_top_1_percent': False, 'is_in_top_10_percent': False}, 'cited_by_percentile_year': {'min': 90, 'max': 91}, 'biblio': {'volume': '13', 'issue': '4', 'first_page': '2441', 'last_page': '2455'}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T10604', 'display_name': 'RNA Research and Splicing', 'score': 0.9996, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, 'topics': [{'id': 'https://openalex.org/T10604', 'display_name': 'RNA Research and Splicing', 'score': 0.9996, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T10521', 'display_name': 'RNA and protein synthesis mechanisms', 'score': 0.9996, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T10497', 'display_name': 'Fungal and yeast genetics research', 'score': 0.9974, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/fibrillarin', 'display_name': 'Fibrillarin', 'score': 0.892532}, {'id': 'https://openalex.org/keywords/rna-polymerase-i', 'display_name': 'RNA polymerase I', 'score': 0.7227013}, {'id': 'https://openalex.org/keywords/rna-polymerase-iii', 'display_name': 'RNA polymerase III', 'score': 0.70926523}, {'id': 'https://openalex.org/keywords/rna-polymerase-ii', 'display_name': 'RNA polymerase II', 'score': 0.47264758}], 'concepts': [{'id': 'https://openalex.org/C183873130', 'wikidata': 'https://www.wikidata.org/wiki/Q30869', 'display_name': 'Nucleolus', 'level': 3, 'score': 0.9159424}, {'id': 'https://openalex.org/C2776007122', 'wikidata': 'https://www.wikidata.org/wiki/Q14914390', 'display_name': 'Fibrillarin', 'level': 4, 'score': 0.892532}, {'id': 'https://openalex.org/C86803240', 'wikidata': 'https://www.wikidata.org/wiki/Q420', 'display_name': 'Biology', 'level': 0, 'score': 0.8440733}, {'id': 'https://openalex.org/C25281209', 'wikidata': 'https://www.wikidata.org/wiki/Q3502188', 'display_name': 'RNA polymerase I', 'level': 5, 'score': 0.7227013}, {'id': 'https://openalex.org/C70916841', 'wikidata': 'https://www.wikidata.org/wiki/Q3501658', 'display_name': 'RNA polymerase III', 'level': 5, 'score': 0.70926523}, {'id': 'https://openalex.org/C67905577', 'wikidata': 'https://www.wikidata.org/wiki/Q215980', 'display_name': 'Ribosomal RNA', 'level': 3, 'score': 0.587941}, {'id': 'https://openalex.org/C2777576037', 'wikidata': 'https://www.wikidata.org/wiki/Q719725', 'display_name': 'Saccharomyces cerevisiae', 'level': 3, 'score': 0.5791103}, {'id': 'https://openalex.org/C153911025', 'wikidata': 'https://www.wikidata.org/wiki/Q7202', 'display_name': 'Molecular biology', 'level': 1, 'score': 0.5200286}, {'id': 'https://openalex.org/C143065580', 'wikidata': 'https://www.wikidata.org/wiki/Q3285695', 'display_name': 'Mutant', 'level': 3, 'score': 0.5142052}, {'id': 'https://openalex.org/C67705224', 'wikidata': 'https://www.wikidata.org/wiki/Q11053', 'display_name': 'RNA', 'level': 3, 'score': 0.50383204}, {'id': 'https://openalex.org/C64350747', 'wikidata': 'https://www.wikidata.org/wiki/Q15334993', 'display_name': 'RNA polymerase II', 'level': 5, 'score': 0.47264758}, {'id': 'https://openalex.org/C2776449523', 'wikidata': 'https://www.wikidata.org/wiki/Q272631', 'display_name': 'RNA polymerase', 'level': 4, 'score': 0.4167721}, {'id': 'https://openalex.org/C95444343', 'wikidata': 'https://www.wikidata.org/wiki/Q7141', 'display_name': 'Cell biology', 'level': 1, 'score': 0.39093512}, {'id': 'https://openalex.org/C104317684', 'wikidata': 'https://www.wikidata.org/wiki/Q7187', 'display_name': 'Gene', 'level': 2, 'score': 0.3503504}, {'id': 'https://openalex.org/C54355233', 'wikidata': 'https://www.wikidata.org/wiki/Q7162', 'display_name': 'Genetics', 'level': 1, 'score': 0.22330081}, {'id': 'https://openalex.org/C150194340', 'wikidata': 'https://www.wikidata.org/wiki/Q26972', 'display_name': 'Gene expression', 'level': 3, 'score': 0.20964202}, {'id': 'https://openalex.org/C101762097', 'wikidata': 'https://www.wikidata.org/wiki/Q224093', 'display_name': 'Promoter', 'level': 4, 'score': 0.14415297}, {'id': 'https://openalex.org/C2780723820', 'wikidata': 'https://www.wikidata.org/wiki/Q1934178', 'display_name': 'Nucleus', 'level': 2, 'score': 0.12798917}], 'mesh': [{'descriptor_ui': 'D002466', 'descriptor_name': 'Cell Nucleolus', 'qualifier_ui': 'Q000648', 'qualifier_name': 'ultrastructure', 'is_major_topic': True}, {'descriptor_ui': 'D012318', 'descriptor_name': 'RNA Polymerase I', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': True}, {'descriptor_ui': 'D012335', 'descriptor_name': 'RNA, Ribosomal', 'qualifier_ui': 'Q000096', 'qualifier_name': 'biosynthesis', 'is_major_topic': True}, {'descriptor_ui': 'D012441', 'descriptor_name': 'Saccharomyces cerevisiae', 'qualifier_ui': 'Q000648', 'qualifier_name': 'ultrastructure', 'is_major_topic': True}, {'descriptor_ui': 'D002466', 'descriptor_name': 'Cell Nucleolus', 'qualifier_ui': 'Q000201', 'qualifier_name': 'enzymology', 'is_major_topic': False}, {'descriptor_ui': 'D002466', 'descriptor_name': 'Cell Nucleolus', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D005800', 'descriptor_name': 'Genes, Fungal', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D012318', 'descriptor_name': 'RNA Polymerase I', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D012331', 'descriptor_name': 'RNA, Fungal', 'qualifier_ui': 'Q000096', 'qualifier_name': 'biosynthesis', 'is_major_topic': False}, {'descriptor_ui': 'D012331', 'descriptor_name': 'RNA, Fungal', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D012335', 'descriptor_name': 'RNA, Ribosomal', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D012441', 'descriptor_name': 'Saccharomyces cerevisiae', 'qualifier_ui': 'Q000201', 'qualifier_name': 'enzymology', 'is_major_topic': False}, {'descriptor_ui': 'D012441', 'descriptor_name': 'Saccharomyces cerevisiae', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D017384', 'descriptor_name': 'Sequence Deletion', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}], 'locations_count': 2, 'locations': [{'is_oa': False, 'landing_page_url': 'https://doi.org/10.1128/mcb.13.4.2441-2455.1993', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S81455967', 'display_name': 'Molecular and Cellular Biology', 'issn_l': '0270-7306', 'issn': ['0270-7306', '1067-8824', '1098-5549'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320547', 'host_organization_name': 'Taylor & Francis', 'host_organization_lineage': ['https://openalex.org/P4310320547'], 'host_organization_lineage_names': ['Taylor & Francis'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, {'is_oa': False, 'landing_page_url': 'https://pubmed.ncbi.nlm.nih.gov/8455621', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306525036', 'display_name': 'PubMed', 'issn_l': None, 'issn': None, 'is_oa': False, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': None, 'sustainable_development_goals': [{'display_name': 'Life on land', 'id': 'https://metadata.un.org/sdg/15', 'score': 0.53}], 'grants': [], 'datasets': [], 'versions': [], 'referenced_works_count': 49, 'referenced_works': ['https://openalex.org/W109701900', 'https://openalex.org/W1542844327', 'https://openalex.org/W1549536215', 'https://openalex.org/W1561300955', 'https://openalex.org/W1608303629', 'https://openalex.org/W1729152903', 'https://openalex.org/W1886218695', 'https://openalex.org/W1939013880', 'https://openalex.org/W1946529825', 'https://openalex.org/W1965316114', 'https://openalex.org/W1967419673', 'https://openalex.org/W1969107860', 'https://openalex.org/W1975268309', 'https://openalex.org/W1975969667', 'https://openalex.org/W1977509652', 'https://openalex.org/W1984491679', 'https://openalex.org/W1988134822', 'https://openalex.org/W1996468935', 'https://openalex.org/W2009859763', 'https://openalex.org/W2016152543', 'https://openalex.org/W2045351722', 'https://openalex.org/W2054342610', 'https://openalex.org/W2059234508', 'https://openalex.org/W2059951698', 'https://openalex.org/W2061372853', 'https://openalex.org/W2065923747', 'https://openalex.org/W2067596993', 'https://openalex.org/W2069725464', 'https://openalex.org/W2072185974', 'https://openalex.org/W2073474487', 'https://openalex.org/W2073788949', 'https://openalex.org/W2080703776', 'https://openalex.org/W2084345643', 'https://openalex.org/W2108840105', 'https://openalex.org/W2122216998', 'https://openalex.org/W2124682700', 'https://openalex.org/W2143240659', 'https://openalex.org/W2158989625', 'https://openalex.org/W2163638358', 'https://openalex.org/W2169142563', 'https://openalex.org/W21943246', 'https://openalex.org/W2323037227', 'https://openalex.org/W2482729339', 'https://openalex.org/W260776168', 'https://openalex.org/W308486584', 'https://openalex.org/W37276328', 'https://openalex.org/W4232938710', 'https://openalex.org/W4248302271', 'https://openalex.org/W4255165284'], 'related_works': ['https://openalex.org/W4386096148', 'https://openalex.org/W4246058695', 'https://openalex.org/W2802948734', 'https://openalex.org/W2462973229', 'https://openalex.org/W2141676283', 'https://openalex.org/W2132623938', 'https://openalex.org/W2087846512', 'https://openalex.org/W1987933963', 'https://openalex.org/W1507809280', 'https://openalex.org/W1485401273'], 'abstract_inverted_index': {'We': [0], 'have': [1], 'previously': [2], 'constructed': [3], 'mutants': [4, 121], 'of': [5, 16, 39, 107, 142, 159, 167, 173], 'Saccharomyces': [6], 'cerevisiae': [7], 'in': [8, 57, 86, 103, 117, 133, 170, 186, 226, 229], 'which': [9], 'the': [10, 13, 40, 47, 69, 79, 104, 108, 143, 160, 165, 171, 174, 204, 230], 'gene': [11, 37], 'for': [12, 212], 'second-largest': [14], 'subunit': [15], 'RNA': [17, 31], 'polymerase': [18, 32], 'I': [19, 119, 169, 232], '(Pol': [20], 'I)': [21], 'is': [22, 28, 84, 208], 'deleted.': [23], 'In': [24], 'these': [25, 87, 99], 'mutants,': [26], 'rRNA': [27, 42, 153, 197, 213, 227], 'synthesized': [29], 'by': [30], 'II': [33], 'from': [34], 'a': [35, 51, 140, 180, 183, 192], 'hybrid': [36], 'consisting': [38], '35S': [41], 'coding': [43], 'region': [44], 'fused': [45], 'to': [46, 112, 162, 188, 195], 'GAL7': [48], 'promoter': [49], 'on': [50], 'plasmid.': [52], 'These': [53, 125], 'strains': [54], 'thus': [55], 'grow': [56], 'galactose': [58], 'but': [59], 'not': [60, 156, 209], 'glucose': [61], 'media.': [62], 'By': [63], 'immunofluorescence': [64], 'microscopy': [65], 'using': [66], 'antibodies': [67, 100], 'against': [68], 'known': [70], 'nucleolar': [71, 82, 110, 176, 206], 'proteins': [72], 'SSB1': [73], 'and': [74, 137, 220], 'fibrillarin,': [75], 'we': [76], 'found': [77], 'that': [78, 96, 151, 203, 221], 'intact': [80, 109, 175, 205], 'crescent-shaped': [81], 'structure': [83, 111, 177, 207], 'absent': [85], 'mutants;': [88], 'instead,': [89], 'several': [90], 'granules': [91], '(called': [92], 'mininucleolar': [93], 'bodies': [94, 131], '[MNBs])': [95], 'stained': [97], 'with': [98], 'were': [101], 'seen': [102], 'nucleus.': [105], 'Conversion': [106], 'MNBs': [113, 126, 222], 'was': [114], 'also': [115, 201], 'observed': [116, 132], 'Pol': [118, 168, 231], 'temperature-sensitive': [120], 'at': [122], 'nonpermissive': [123], 'temperatures.': [124], 'may': [127, 138], 'structurally': [128], 'resemble': [129], 'prenucleolar': [130], 'higher': [134], 'eukaryotic': [135], 'cells': [136, 147], 'represent': [139], 'constituent': [141], 'normal': [144], 'nucleolus.': [145], 'Furthermore,': [146], 'under': [148], 'certain': [149], 'conditions': [150], 'inhibit': [152], 'synthesis': [154], 'did': [155], 'cause': [157], 'conversion': [158], 'nucleolus': [161], 'MNBs.': [163], 'Thus,': [164], 'role': [166, 181, 194], 'maintenance': [172], 'might': [178], 'include': [179], 'as': [182], 'structural': [184], 'element': [185], 'addition': [187], '(or': [189], 'instead': [190], 'of)': [191], 'functional': [193, 225], 'produce': [196], 'transcripts.': [198], 'Our': [199], 'study': [200], 'shows': [202], 'absolutely': [210], 'required': [211], 'processing,': [214], 'ribosome': [215], 'assembly,': [216], 'or': [217], 'cell': [218], 'growth': [219], 'are': [223], 'possibly': [224], 'processing': [228], 'deletion': [233], 'mutants.': [234]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W4246058695', 'counts_by_year': [{'year': 2023, 'cited_by_count': 1}, {'year': 2019, 'cited_by_count': 1}, {'year': 2018, 'cited_by_count': 2}, {'year': 2017, 'cited_by_count': 2}, {'year': 2014, 'cited_by_count': 1}, {'year': 2013, 'cited_by_count': 1}], 'updated_date': '2024-12-16T02:42:13.464004', 'created_date': '2022-05-12'}