Title: Purification and characterization of restriction endonuclease BcoI from a soil isolate of Bacillus coagulans
Abstract: A soil identified as Bacillus coagulans is found to produce BcoI, an isoschizomer of AvaI and with the same cleavage site. This thermal stable enzyme, BcoI, is produced at high level and can be isolated by passing the crude bacterial lysate through a DEAE-cellulose column.