Title: Tunicamycin aggravates cell migration, cell invasion and cell proliferation in colonic epithelial cells
Abstract: Abstract Aim : Endoplasmic reticular stress (ERS) has been well-documented in escalating metastasis in clinical conditions. There has been a debate on its role in cancer portraying it as a double-edged sword. However, there is no evidence of increased cell migration and cell invasion under increased ERS by tunicamycin(TUN) 10μg/mL. Methods : In this study, we treated colonic epithelial cells (LS174T cells) with TUN and measured cell proliferation (by Ki67+ assay), cell migration and cell invasion at the 6 th hour specifically. Results : Our results have demonstrated a positive correlation between TUN and cell migration, cell invasion, and proliferation. TUN treatment has significantly escalated cell invasion, migration and proliferation in LS174T cells. Conclusion : These preliminary results clearly suggest that TUN might promote metastasis of gastrointestinal cancers.