Title: Effect of lipid supplements on the production and glycosylation of recombinant interferon-γ expressed in CHO cells
Abstract: The effects of lipids on the glycosylation of recombinant human interferon-γ expressed in a Chinese Hamster Ovary cell line were investigated in batch culture. Lipids form an essential part of the N-glycosylation pathway, and have been shown to improve cell viability. In control (serum-free) medium the proportion of fully-glycosylated interferon-γ deteriorated reproducibly with time in batch culture, but the lipoprotein supplement ExCyte was shown to minimise this trend. Partially substituting the bovine serum albumin content of the medium with a fatty-acid free preparation also improved interferon-γ glycosylation, possibly indicating that oxidised lipids carried on Cohn fraction V albumin may damage the glycosylation process.
Publication Year: 1994
Publication Date: 1994-01-01
Language: en
Type: book-chapter
Indexed In: ['crossref']
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Cited By Count: 2
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