Title: Metabolic Phytochemistry-Based Approaches for Studying Secondary Metabolism Using Transformed Root Culture Systems
Abstract: Though secondary metabolism is integrated in networks with primary metabolism, still the biosynthetic pathways for most of the structurally known plant secondary compounds are unknown. Transformed roots developed following integration and expression of genes from Agrobacterium rhizogenes have proved to be a valuable model system in studying secondary metabolic pathways since they generally reflect the in planta operation of these pathways both in their route and enzymology. Transformed root culture is an isolated organ-culture system and physiologically distinct from the whole-plant system. This is important on two counts. First, transformed root cultures offer a better and simpler system in which to relate enzyme activities and product formation, uncomplicated by potential transport and source-sink relationships. Secondly, there is scant information in literature concerning phenylpropanoid and terpenoid metabolisms in roots of any kind. In this chapter, we report our findings with transformed root cultures of Daucus carota on a new biochemical pathway of hydroxybenzoic acid biosynthesis with an evidence of a phenylpropanoid chain cleavage enzyme for benzenoid formation and diversion of metabolic flows from phenylpropanoids to isoprenoids in green transformed roots upon photooxidative stress. Metabolite profiling using HPLC/ESI-MS/GC-MS, HPLC-based in vitro enzyme assays, inhibitor feeding experiments, and transcript analysis formed the basis of the above investigations.
Publication Year: 2017
Publication Date: 2017-01-01
Language: en
Type: book-chapter
Indexed In: ['crossref']
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Cited By Count: 2
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