Abstract: This chapter discusses the procedures that specifically involve organelle transfer or organelle-controlled traits and reviews the techniques for isolation, culture, fusion of protoplasts and plant regeneration from cultured protoplasts. Organelle transmission in most of the higher plants is uniparental. Thus, chloroplasts and mitochondria are transmitted together, maternally, irrespective of if the plants are self- or cross-pollinated. Transfer of heritable traits by conventional genetic manipulation, as practiced for nuclear genes, is therefore not applicable to organelle genes. To facilitate the construction of a plant having in its cells a given nuclear genome but chloroplast and/or mitochondria from another plant, a donor-recipient fusion should be performed. The cybrid plants, derived from such a fusion, should result from a Recipient protoplast where the original functional nucleus is retained, but either one or both of the cytoplasmic organelles––that is, chloroplasts and mitochondria––is exchanged by organelles from a Donor plant.
Publication Year: 1986
Publication Date: 1986-01-01
Language: en
Type: book-chapter
Indexed In: ['crossref']
Access and Citation
Cited By Count: 42
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot