Title: Mapping and identification of HeLa cell proteins separated by immobilized pH-gradient two-dimensional gel electrophoresis and construction of a two-dimensional polyacrylamide gel electrophoresis database
Abstract: ELECTROPHORESISVolume 20, Issue 4-5 p. 977-983 Research Article Mapping and identification of HeLa cell proteins separated by immobilized pH-gradient two-dimensional gel electrophoresis and construction of a two-dimensional polyacrylamide gel electrophoresis database Allan Christian Shaw, Corresponding Author Allan Christian Shaw [email protected] Department of Medical Microbiology and Immunology, University of Aarhus, Denmark Department of Molecular and Structural Biology, University of Aarhus, DenmarkDepartment of Medical Microbiology and Immunology, The Bartholin Building, University of Aarhus, DK-8000 Aarhus C, Denmark, Fax: +45-8619-6128===Search for more papers by this authorMartin Røssel Larsen, Martin Røssel Larsen Department of Molecular Biology, University of Odense, DenmarkSearch for more papers by this authorPeter Roepstorff, Peter Roepstorff Department of Molecular Biology, University of Odense, DenmarkSearch for more papers by this authorArne Holm, Arne Holm The Royal Veterinary and Agricultural University, Copenhagen, DenmarkSearch for more papers by this authorGunna Christiansen, Gunna Christiansen Department of Medical Microbiology and Immunology, University of Aarhus, DenmarkSearch for more papers by this authorSvend Birkelund, Svend Birkelund Department of Medical Microbiology and Immunology, University of Aarhus, DenmarkSearch for more papers by this author Allan Christian Shaw, Corresponding Author Allan Christian Shaw [email protected] Department of Medical Microbiology and Immunology, University of Aarhus, Denmark Department of Molecular and Structural Biology, University of Aarhus, DenmarkDepartment of Medical Microbiology and Immunology, The Bartholin Building, University of Aarhus, DK-8000 Aarhus C, Denmark, Fax: +45-8619-6128===Search for more papers by this authorMartin Røssel Larsen, Martin Røssel Larsen Department of Molecular Biology, University of Odense, DenmarkSearch for more papers by this authorPeter Roepstorff, Peter Roepstorff Department of Molecular Biology, University of Odense, DenmarkSearch for more papers by this authorArne Holm, Arne Holm The Royal Veterinary and Agricultural University, Copenhagen, DenmarkSearch for more papers by this authorGunna Christiansen, Gunna Christiansen Department of Medical Microbiology and Immunology, University of Aarhus, DenmarkSearch for more papers by this authorSvend Birkelund, Svend Birkelund Department of Medical Microbiology and Immunology, University of Aarhus, DenmarkSearch for more papers by this author First published: 27 April 1999 https://doi.org/10.1002/(SICI)1522-2683(19990101)20:4/5<977::AID-ELPS977>3.0.CO;2-JCitations: 20AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract The HeLa cell line, a human adenocarcinoma, is used in many research fields, since it can be infected with a wide range of viruses and intracellular bacteria. Therefore, the mapping of HeLa cell proteins is useful for the investigation of parasite host cell interactions. Because of the recent improvements of two-dimensional gel electrophoresis with immobilized pH gradients (IPG) compared to isoelectric focusing with carrier ampholytes, a highly reproducible method for examining global changes in HeLa cell protein expression due to different stimuli is now available. Therefore, we have initiated the mapping of [35S]methionine/cysteine-labeled HeLa cell proteins with the 2-D PAGE (IPG)-system, using matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS) and N-terminal sequencing for protein identification. To date 21 proteins have been identified and mapped. 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Publication Year: 1999
Publication Date: 1999-01-01
Language: en
Type: article
Indexed In: ['crossref']
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Cited By Count: 1
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