Title: In Vitro Efficacy of Lufenuron against Filamentous Fungi and Blood Concentrations after PO Administration in Horses
Abstract: Journal of Veterinary Internal MedicineVolume 19, Issue 6 p. 878-882 Open Access In Vitro Efficacy of Lufenuron against Filamentous Fungi and Blood Concentrations after PO Administration in Horses Nicole C. Scotty, Corresponding Author Nicole C. Scotty Departments of Veterinary Medicine and Surgery Veterinary Pathobiology DVM, Florida Veterinary Specialists, 3000 Busch Lake Boulevard, Tampa, FL 33614; e-mail: [email protected] for more papers by this authorTim J. Evans, Tim J. Evans College of Veterinary Medicine, University of Missouri, Columbia, MOSearch for more papers by this authorElizabeth Giuliano, Elizabeth Giuliano Departments of Veterinary Medicine and Surgery Veterinary PathobiologySearch for more papers by this authorPhilip J. Johnson, Philip J. Johnson Departments of Veterinary Medicine and Surgery Veterinary PathobiologySearch for more papers by this authorGeorge E. Rottinghaus, George E. Rottinghaus College of Veterinary Medicine, University of Missouri, Columbia, MOSearch for more papers by this authorAnnette W. Fothergill, Annette W. Fothergill Department of Pathology, University of Texas Health Science Center at San Antonio, San Antonio, TXSearch for more papers by this authorTim J. Cutler, Tim J. Cutler Animal Eye Specialty Clinic, West Palm Beach, FLSearch for more papers by this author Nicole C. Scotty, Corresponding Author Nicole C. Scotty Departments of Veterinary Medicine and Surgery Veterinary Pathobiology DVM, Florida Veterinary Specialists, 3000 Busch Lake Boulevard, Tampa, FL 33614; e-mail: [email protected] for more papers by this authorTim J. Evans, Tim J. Evans College of Veterinary Medicine, University of Missouri, Columbia, MOSearch for more papers by this authorElizabeth Giuliano, Elizabeth Giuliano Departments of Veterinary Medicine and Surgery Veterinary PathobiologySearch for more papers by this authorPhilip J. Johnson, Philip J. Johnson Departments of Veterinary Medicine and Surgery Veterinary PathobiologySearch for more papers by this authorGeorge E. Rottinghaus, George E. Rottinghaus College of Veterinary Medicine, University of Missouri, Columbia, MOSearch for more papers by this authorAnnette W. Fothergill, Annette W. Fothergill Department of Pathology, University of Texas Health Science Center at San Antonio, San Antonio, TXSearch for more papers by this authorTim J. Cutler, Tim J. Cutler Animal Eye Specialty Clinic, West Palm Beach, FLSearch for more papers by this author First published: 28 June 2008 https://doi.org/10.1111/j.1939-1676.2005.tb02781.xCitations: 10AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract Lufenuron is a benzoylphenyl urea-derived insecticide that has been recently introduced as a novel treatment for fungal infections in horses. The purposes of this study were to determine (1) the in vitro efficacy of lufenuron against Aspergillus and Fusarium spp. and (2) the ability of lufenuron to reach efficacious blood concentrations after PO administration in horses. Fungal colonies isolated from diseased equine corneas were tested against lufenuron solutions up to 700 μg/mL. Twenty-one adult horses received 1 of 3 PO lufenuron treatment regimens: 5 mg/kg body weight (BW) q24h for 3 days, 20 mg/kg BW q24h for 3 days, or 60 mg/kg BW q24h for 1 day. Blood samples were collected up to 96 hours after drug administration and analyzed by high-performance liquid chromatography. Statistical analyses of lufenuron blood concentrations were performed by analysis of variance and Fischer's Least Significant Difference test, with statistical significance set at P < .05. Lufenuron showed no effect on the in vitro growth of Aspergillus or Fusarium spp. Lufenuron was detected in the blood of all but 1 horse and showed no adverse effects. The maximum blood lufenuron concentration (83.5 ± 58.7 μg/L) was lower than the concentrations proven to be ineffective in vitro in this study. Further therapeutic use of lufenuron as an antifungal agent in horses should be based on proven efficacy against specific strains of clinically relevant fungi with pharmacokinetic data demonstrating sufficient lufenuron concentrations in target tissues. References 1 Hess MB, Parker NA, Purswell BJ, et al. Use of lufenuron as a treatment for fungal endometritis in four mares. J Am Vet Med Assoc 2002; 15: 266– 267. 2 Cohen E. Interference with chitin biosynthesis in insects. In: JE Wright, A. Retnakaran, eds. Chitin and Benzoylphenyl Ureas, Series Entomologica, Vol 38. Boston , MA : Dr W Junk Publishers; 1987: 33– 42. 3 Ben-Ziony Y., Arzi B. 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