Cloning and expression of a GH11 xylanase from Bacillus pumilus SSP-34 in Pichia pastoris GS115: Purification and characterization

Name: Work Video:
Duration: 3 min 30 s
Description: Expression analysis of recombinant Vigna radiata plant defensin 1 protein in transgenic tobacco plantsHoang Thi Thao , Nguyen Thi Ngoc Lan, Ho Manh Tuong, Nguyen Vu Thanh Thanh, Le Van Son, Chu Hoang Mau
Bhat Sagar Krishna; Kavya Purushothaman; Appu Rao Appu Rao Gopala Rao; Kini Kukkundoor Ramachandra
{'id': 'https://openalex.org/W4213329479', 'doi': 'https://doi.org/10.7324/jabb.2022.100215', 'title': 'Cloning and expression of a GH11 xylanase from Bacillus pumilus SSP-34 in Pichia pastoris GS115: Purification and characterization', 'display_name': 'Cloning and expression of a GH11 xylanase from Bacillus pumilus SSP-34 in Pichia pastoris GS115: Purification and characterization', 'publication_year': 2022, 'publication_date': '2022-02-15', 'ids': {'openalex': 'https://openalex.org/W4213329479', 'doi': 'https://doi.org/10.7324/jabb.2022.100215'}, 'language': 'en', 'primary_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.7324/jabb.2022.100215', 'pdf_url': 'https://jabonline.in/admin/php/uploads/697_pdf.pdf', 'source': {'id': 'https://openalex.org/S4210205073', 'display_name': 'Journal of Applied Biology & Biotechnology', 'issn_l': '2347-212X', 'issn': ['2347-212X', '2455-7005'], 'is_oa': True, 'is_in_doaj': False, 'is_core': True, 'host_organization': None, 'host_organization_name': None, 'host_organization_lineage': [], 'host_organization_lineage_names': [], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': ['crossref'], 'open_access': {'is_oa': True, 'oa_status': 'gold', 'oa_url': 'https://jabonline.in/admin/php/uploads/697_pdf.pdf', 'any_repository_has_fulltext': False}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5068782985', 'display_name': 'Bhat Sagar Krishna', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I4210100303', 'display_name': 'Biotech Park', 'ror': 'https://ror.org/00mv0vt78', 'country_code': 'IN', 'type': 'government', 'lineage': ['https://openalex.org/I4210100303']}, {'id': 'https://openalex.org/I204743663', 'display_name': 'University of Mysore', 'ror': 'https://ror.org/012bxv356', 'country_code': 'IN', 'type': 'education', 'lineage': ['https://openalex.org/I204743663']}], 'countries': ['IN'], 'is_corresponding': False, 'raw_author_name': 'Bhat Sagar Krishna', 'raw_affiliation_strings': ['Department of Studies in Biotechnology, University of Mysore, Mysuru, India.', 'Kaypeeyes Biotech Pvt Ltd, RD Center, Hebbal Industrial Area, Mysuru, India.'], 'affiliations': [{'raw_affiliation_string': 'Kaypeeyes Biotech Pvt Ltd, RD Center, Hebbal Industrial Area, Mysuru, India.', 'institution_ids': ['https://openalex.org/I4210100303']}, {'raw_affiliation_string': 'Department of Studies in Biotechnology, University of Mysore, Mysuru, India.', 'institution_ids': ['https://openalex.org/I204743663']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5061720669', 'display_name': 'Kavya Purushothaman', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I4210100303', 'display_name': 'Biotech Park', 'ror': 'https://ror.org/00mv0vt78', 'country_code': 'IN', 'type': 'government', 'lineage': ['https://openalex.org/I4210100303']}], 'countries': ['IN'], 'is_corresponding': False, 'raw_author_name': 'Purushothaman Kavya', 'raw_affiliation_strings': ['Kaypeeyes Biotech Pvt Ltd, RD Center, Hebbal Industrial Area, Mysuru, India.'], 'affiliations': [{'raw_affiliation_string': 'Kaypeeyes Biotech Pvt Ltd, RD Center, Hebbal Industrial Area, Mysuru, India.', 'institution_ids': ['https://openalex.org/I4210100303']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5075111337', 'display_name': 'Appu Rao Appu Rao Gopala Rao', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I4210100303', 'display_name': 'Biotech Park', 'ror': 'https://ror.org/00mv0vt78', 'country_code': 'IN', 'type': 'government', 'lineage': ['https://openalex.org/I4210100303']}], 'countries': ['IN'], 'is_corresponding': False, 'raw_author_name': 'Appu Rao Appu Rao Gopala Rao', 'raw_affiliation_strings': ['Kaypeeyes Biotech Pvt Ltd, RD Center, Hebbal Industrial Area, Mysuru, India.'], 'affiliations': [{'raw_affiliation_string': 'Kaypeeyes Biotech Pvt Ltd, RD Center, Hebbal Industrial Area, Mysuru, India.', 'institution_ids': ['https://openalex.org/I4210100303']}]}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5091450235', 'display_name': 'Kini Kukkundoor Ramachandra', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I204743663', 'display_name': 'University of Mysore', 'ror': 'https://ror.org/012bxv356', 'country_code': 'IN', 'type': 'education', 'lineage': ['https://openalex.org/I204743663']}], 'countries': ['IN'], 'is_corresponding': False, 'raw_author_name': 'Kini K Ramachandra', 'raw_affiliation_strings': ['Department of Studies in Biotechnology, University of Mysore, Mysuru, India.'], 'affiliations': [{'raw_affiliation_string': 'Department of Studies in Biotechnology, University of Mysore, Mysuru, India.', 'institution_ids': ['https://openalex.org/I204743663']}]}], 'institution_assertions': [], 'countries_distinct_count': 1, 'institutions_distinct_count': 2, 'corresponding_author_ids': [], 'corresponding_institution_ids': [], 'apc_list': None, 'apc_paid': None, 'fwci': 0.409, 'has_fulltext': True, 'fulltext_origin': 'pdf', 'cited_by_count': 2, 'citation_normalized_percentile': {'value': 0.653031, 'is_in_top_1_percent': False, 'is_in_top_10_percent': False}, 'cited_by_percentile_year': {'min': 70, 'max': 76}, 'biblio': {'volume': None, 'issue': None, 'first_page': None, 'last_page': None}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T12856', 'display_name': 'Transgenic Plants and Applications', 'score': 0.997, 'subfield': {'id': 'https://openalex.org/subfields/1305', 'display_name': 'Biotechnology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, 'topics': [{'id': 'https://openalex.org/T12856', 'display_name': 'Transgenic Plants and Applications', 'score': 0.997, 'subfield': {'id': 'https://openalex.org/subfields/1305', 'display_name': 'Biotechnology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T10240', 'display_name': 'Plant tissue culture and regeneration', 'score': 0.9877, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T12683', 'display_name': 'Studies on Chitinases and Chitosanases', 'score': 0.984, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/bacillus-pumilus', 'display_name': 'Bacillus pumilus', 'score': 0.8764375}, {'id': 'https://openalex.org/keywords/cloning', 'display_name': 'Cloning (programming)', 'score': 0.6272005}, {'id': 'https://openalex.org/keywords/radiata', 'display_name': 'Radiata', 'score': 0.45295393}], 'concepts': [{'id': 'https://openalex.org/C2778187402', 'wikidata': 'https://www.wikidata.org/wiki/Q4838979', 'display_name': 'Bacillus pumilus', 'level': 3, 'score': 0.8764375}, {'id': 'https://openalex.org/C2781372317', 'wikidata': 'https://www.wikidata.org/wiki/Q148697', 'display_name': 'Pichia pastoris', 'level': 4, 'score': 0.7799911}, {'id': 'https://openalex.org/C86803240', 'wikidata': 'https://www.wikidata.org/wiki/Q420', 'display_name': 'Biology', 'level': 0, 'score': 0.7115532}, {'id': 'https://openalex.org/C121050878', 'wikidata': 'https://www.wikidata.org/wiki/Q5135020', 'display_name': 'Cloning (programming)', 'level': 2, 'score': 0.6272005}, {'id': 'https://openalex.org/C40767141', 'wikidata': 'https://www.wikidata.org/wiki/Q285697', 'display_name': 'Recombinant DNA', 'level': 3, 'score': 0.5717171}, {'id': 'https://openalex.org/C2779862049', 'wikidata': 'https://www.wikidata.org/wiki/Q132634', 'display_name': 'Vigna', 'level': 2, 'score': 0.48565733}, {'id': 'https://openalex.org/C2778357721', 'wikidata': 'https://www.wikidata.org/wiki/Q719268', 'display_name': 'Radiata', 'level': 3, 'score': 0.45295393}, {'id': 'https://openalex.org/C59822182', 'wikidata': 'https://www.wikidata.org/wiki/Q441', 'display_name': 'Botany', 'level': 1, 'score': 0.4164763}, {'id': 'https://openalex.org/C153911025', 'wikidata': 'https://www.wikidata.org/wiki/Q7202', 'display_name': 'Molecular biology', 'level': 1, 'score': 0.36214337}, {'id': 'https://openalex.org/C55493867', 'wikidata': 'https://www.wikidata.org/wiki/Q7094', 'display_name': 'Biochemistry', 'level': 1, 'score': 0.1968503}, {'id': 'https://openalex.org/C54355233', 'wikidata': 'https://www.wikidata.org/wiki/Q7162', 'display_name': 'Genetics', 'level': 1, 'score': 0.17536908}, {'id': 'https://openalex.org/C104317684', 'wikidata': 'https://www.wikidata.org/wiki/Q7187', 'display_name': 'Gene', 'level': 2, 'score': 0.17211491}, {'id': 'https://openalex.org/C523546767', 'wikidata': 'https://www.wikidata.org/wiki/Q10876', 'display_name': 'Bacteria', 'level': 2, 'score': 0.09795588}, {'id': 'https://openalex.org/C41008148', 'wikidata': 'https://www.wikidata.org/wiki/Q21198', 'display_name': 'Computer science', 'level': 0, 'score': 0.0}, {'id': 'https://openalex.org/C199360897', 'wikidata': 'https://www.wikidata.org/wiki/Q9143', 'display_name': 'Programming language', 'level': 1, 'score': 0.0}], 'mesh': [], 'locations_count': 1, 'locations': [{'is_oa': True, 'landing_page_url': 'https://doi.org/10.7324/jabb.2022.100215', 'pdf_url': 'https://jabonline.in/admin/php/uploads/697_pdf.pdf', 'source': {'id': 'https://openalex.org/S4210205073', 'display_name': 'Journal of Applied Biology & Biotechnology', 'issn_l': '2347-212X', 'issn': ['2347-212X', '2455-7005'], 'is_oa': True, 'is_in_doaj': False, 'is_core': True, 'host_organization': None, 'host_organization_name': None, 'host_organization_lineage': [], 'host_organization_lineage_names': [], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}], 'best_oa_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.7324/jabb.2022.100215', 'pdf_url': 'https://jabonline.in/admin/php/uploads/697_pdf.pdf', 'source': {'id': 'https://openalex.org/S4210205073', 'display_name': 'Journal of Applied Biology & Biotechnology', 'issn_l': '2347-212X', 'issn': ['2347-212X', '2455-7005'], 'is_oa': True, 'is_in_doaj': False, 'is_core': True, 'host_organization': None, 'host_organization_name': None, 'host_organization_lineage': [], 'host_organization_lineage_names': [], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'sustainable_development_goals': [{'id': 'https://metadata.un.org/sdg/3', 'display_name': 'Good health and well-being', 'score': 0.74}], 'grants': [], 'datasets': [], 'versions': [], 'referenced_works_count': 23, 'referenced_works': ['https://openalex.org/W1775749144', 'https://openalex.org/W1965020882', 'https://openalex.org/W1974725190', 'https://openalex.org/W1989552928', 'https://openalex.org/W1990667026', 'https://openalex.org/W1991890196', 'https://openalex.org/W2000028565', 'https://openalex.org/W2055566537', 'https://openalex.org/W2079074163', 'https://openalex.org/W2080569295', 'https://openalex.org/W2088534336', 'https://openalex.org/W2100837269', 'https://openalex.org/W2128949999', 'https://openalex.org/W2141157874', 'https://openalex.org/W2152756549', 'https://openalex.org/W2161933333', 'https://openalex.org/W2171035693', 'https://openalex.org/W2744570342', 'https://openalex.org/W2982219949', 'https://openalex.org/W2995566875', 'https://openalex.org/W3138532363', 'https://openalex.org/W3202251530', 'https://openalex.org/W4240316299'], 'related_works': ['https://openalex.org/W4290994333', 'https://openalex.org/W2799981841', 'https://openalex.org/W2523500887', 'https://openalex.org/W2421514097', 'https://openalex.org/W2376656822', 'https://openalex.org/W2376556152', 'https://openalex.org/W230641855', 'https://openalex.org/W2277885220', 'https://openalex.org/W2215161613', 'https://openalex.org/W2102186664'], 'abstract_inverted_index': {'Expression': [0], 'analysis': [1], 'of': [2], 'recombinant': [3], 'Vigna': [4], 'radiata': [5], 'plant': [6], 'defensin': [7], '1': [8], 'protein': [9], 'in': [10], 'transgenic': [11], 'tobacco': [12], 'plantsHoang': [13], 'Thi': [14, 18], 'Thao': [15], ',': [16], 'Nguyen': [17, 24], 'Ngoc': [19], 'Lan,': [20], 'Ho': [21], 'Manh': [22], 'Tuong,': [23], 'Vu': [25], 'Thanh': [26], 'Thanh,': [27], 'Le': [28], 'Van': [29], 'Son,': [30], 'Chu': [31], 'Hoang': [32], 'Mau': [33]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W4213329479', 'counts_by_year': [{'year': 2023, 'cited_by_count': 1}, {'year': 2022, 'cited_by_count': 1}], 'updated_date': '2024-12-13T04:53:46.134571', 'created_date': '2022-02-24'}
Publication Information

Basic Information

Access and Citation

AI Researcher Chatbot

Primary Location

Authors

Topics

Keywords

Related Works
