Title: Isolation and Screening of Symbiotic Actinobacteria from Root Nodules of Actinorhizal Plant Casuarina sp.
Abstract: Actinorhizal plants host mutualistic symbionts of the nitrogen-fixing actinobacterial genus Frankia and non-Frankia plant promoting endophytes within nodule structures formed on their roots. Several plant-growth-promoting endophytic bacteria have also been isolated from actinorhizal root nodules, but little is known about them. Investigating the in planta microbial community composition of actinorhizal root nodules using culture-dependent and culture-independent techniques is helpful for host-specific microbial inoculant development for actinorhizal plant family Casuarinaceae contains the genera Casuarina, Allocasuarina, and Gymnostoma. The screening and selection of potential endophytes for plant growth promotion in waste land reclamation as agroforestry development. This protocol describes the isolation, characterization and identification of the root nodule bacterial endophytes of selected actinorhizal plants by culture-dependent method. Screening the symbiotic ability of non-Frankia and Frankia isolates by in vitro hydrophonic method and selection of potent symbionts for the growth of actinorhizal plant Casuarina sp.
Publication Year: 2022
Publication Date: 2022-01-01
Language: en
Type: book-chapter
Indexed In: ['crossref']
Access and Citation
Cited By Count: 1
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot