Abstract: A detailed understanding of separation science is essential in order to make best use of the wide range of techniques used in analytical toxicology. In chromatography, a suitable flowing mobile phase or eluent is introduced to the mixture or eluent flow is otherwise initiated and the components ofthe mixture are separated by differential physiochemical interaction with the stationary phase. The stationary phase may be a porous solid such as silica gel (adsorption chromatography), or an immiscible or non-volatile liquid held on a suitable solid support (partition chromatography). This chapter discusses the theoretical aspects of gas chromatography and liquid chromatography, including analyte phase distribution, column efficiency, zone broadening, kinetic plots, extra-column contributions, temperature programming and gradient elution, and peak asymmetry. It describes the measurement of analyte retention through planar chromatography and elution chromatography.
Publication Year: 2020
Publication Date: 2020-06-05
Language: en
Type: other
Indexed In: ['crossref']
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