Title: Specific quantification of human genomes from low copy number DNA samples in forensic and ancient DNA studies.
Abstract: We reviewed the current methodologies used for human DNA quantitation in forensic and ancient DNA studies, including sensitive hybridization methods based on the detection of nuclear alpha-satellite repetitive DNA regions or more recently developed fluorogenic real-time polymerase chain reaction (PCR) designs for the detection of both nuclear and mitochondrial DNA regions. Special emphasis has been put on the applicability of recently described different real-time PCR designs targeting different fragments of the HV1 mtDNA control region, and a segment of the X-Y homologous amelogenin gene. The importance of these quantitative assays is to ensure the consistency of low copy number DNA typing (STR profiling and mtDNA sequencing).
Publication Year: 2003
Publication Date: 2003-06-01
Language: en
Type: article
Indexed In: ['pubmed']
Access and Citation
Cited By Count: 66
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot