Title: ANTITUMOR EFFECT OF COMBINED USE OF PURIFIED HUMAN NATURAL TUMOR NECROSIS FACTOR (N-TNF) AND CIS-DIAMMINEDICHOROPLATINUM (II) (CISPLATIN) ON THE IMPLANTED BLADDER CARCINOMA (MBT-2) IN MICE
Abstract: The antitumor effect of tumor necrosis factor (TNF) in combination with Cis-diamminedichloroplatinum (II) (Cisplatin) upon implanted bladder carcinoma (MBT-2) in mice was analysed together with the toxicity. Mice were implanted into the bladder wall with about 104 cells MBT-2 viable cells. TNF was the purified human natural tumor necrosis factor. The antitumor effects were evaluated by the volume of the tumor thus implanted and the drug-toxity by the body weight of mice and microscopic as well as macroscopic findings of the main organs.As a result, 1) The toxity of TNF to mice was not enhanced in a dose-dependant fashion when combined with Cisplatin.2) The antitumor effect by combination of TNF and Cisplatin was significantly increased compared to control group.3) The effects of TNF was enhanced in a dose-dependant fashion when combined with Cisplatin.4) The antitumor effects was confirmed by pathological findings.These results suggest that the combined use of n-TNF and Cisplatin is promising as a clinically effective treatment against bladder cancer in human.