Title: The nucleotide sequence of a complete chicken delta-crystallin cDNA.
Abstract: Research Article1 June 1984free access The nucleotide sequence of a complete chicken delta-crystallin cDNA. K. Yasuda K. Yasuda Search for more papers by this author N. Nakajima N. Nakajima Search for more papers by this author T. Isobe T. Isobe Search for more papers by this author T.S. Okada T.S. Okada Search for more papers by this author Y. Shimura Y. Shimura Search for more papers by this author K. Yasuda K. Yasuda Search for more papers by this author N. Nakajima N. Nakajima Search for more papers by this author T. Isobe T. Isobe Search for more papers by this author T.S. Okada T.S. Okada Search for more papers by this author Y. Shimura Y. Shimura Search for more papers by this author Author Information K. Yasuda, N. Nakajima, T. Isobe, T.S. Okada and Y. Shimura The EMBO Journal (1984)3:1397-1402https://doi.org/10.1002/j.1460-2075.1984.tb01983.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info The nucleotide sequence of a full length cDNA of delta-crystallin mRNA from chicken lens has been determined using a delta-crystallin cDNA clone (pB delta 11), which represents the mRNA sequence of 1530 nucleotides from the poly(A) junction but does not contain the 5′-terminal sequence of 44 nucleotides of the mRNA. The 5′-terminal sequence of the mRNA, absent in the cDNA clone, has been determined with a stretch of cDNA sequence by the primer extension procedure. The amino acid sequence deduced from the nucleotide sequence is consistent with the amino acid sequences of several tryptic peptides, the total amino acid composition, and the mol. wt. of delta-crystallin estimated by SDS-polyacrylamide gel electrophoresis. The computer-assisted analysis predicts high alpha-helical content throughout the polypeptide. Sequence analyses have revealed that gene 1 encodes the mRNA from which the cDNA clone was derived. Previous ArticleNext Article Volume 3Issue 61 June 1984In this issue RelatedDetailsLoading ...