Title: Biodegradation and Decolourization of Orange G by using Bacillus Sp. Isolated from Textile Effluents
Abstract:In many commercial industries the azo dyes are the frequently used dyes. 25 bacterial isolates were taken from the textile waste, and form this 3 bacterial sample (AK 1, AK 2, AK 3) shows the best deg...In many commercial industries the azo dyes are the frequently used dyes. 25 bacterial isolates were taken from the textile waste, and form this 3 bacterial sample (AK 1, AK 2, AK 3) shows the best degradation capacity to degrade Orange G. By observing all the standard morphology and biochemical tests it was observed that these bacteria were the <em>Bacillus Species,</em> and it is found that isolate AK 3 showed the maximum decolourization i.e. 96% within 48 hours. The temperature at which the maximum degradation was observed is at 30°c, the pH was set up to be normal and glucose was used as a carbon source and for the nitrogen source yeast extract was used. The enzymatic activity such as Azo Reductase, Tyrosinase, Lignin Peroxidase (LiP), Manganese Peroxidase (MnP) was observed as their role in biodegradation. This research paper is an analysis of illuminated collection of the benefit of enzymes for decolourization of azo dye and also their utilization for analysis of azo compounds present in waste water.Read More