Title: Genetic diversity evaluation in wild Muntingia calabura L. based on Random Amplified Polymorphic DNA (RAPD) markers
Abstract: This present research was aimed to contribute fundamental knowledge about genetic diversity in wild Muntingia calabura. Random Amplified Polymorphic DNA (RAPD); a method that has been widely used to study genetic relationships in fruit crops. In this research investigation, eight accessions were collected, four accessions from Bangkok, Thailand and four accessions from Medan, North Sumatera, Indonesia. DNA fingerprinting of the eight accessions was performed using twelve RAPD markers (OPA-01, OPA-02, OPA-07, OPA-11, OPC-08, OPC-09, RAPD-01, RAPD-02, RAPD-03, RAPD-04, RAPD-05, RAPD-06). The obtained results revealed that all twelve primers produced polymorphic bands. UPGMA (unweighted pair-group method using arithmetic averages) method was used to determine the genetic distance. Based on dendrogram analysis, the eight accessions of M. calabura formed two main clusters. The range of genetic distance based on RAPD scores was 0.50–0.85. The RAPD technology is suitable for anonymous genomes due to its adaptability to situations with limited quantities of DNA and higher efficiency, and low cost. Researchers hope that present work is impactful and useful for genetic breeding programs in the future.
Publication Year: 2021
Publication Date: 2021-08-28
Language: en
Type: article
Indexed In: ['crossref']
Access and Citation
Cited By Count: 11
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