Title: Development and application of an indirect ELISA for the detection of antibody to porcine circovirus 4 in pigs
Abstract: Transboundary and Emerging DiseasesVolume 68, Issue 6 p. 2975-2979 SPECIAL ISSUE ARTICLE Development and application of an indirect ELISA for the detection of antibody to porcine circovirus 4 in pigs Zhengmin Lian, Zhengmin Lian Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, China These authors contributed equally to this study.Search for more papers by this authorJinbiao Liu, Jinbiao Liu Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, China These authors contributed equally to this study.Search for more papers by this authorPanrao Liu, Panrao Liu Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, ChinaSearch for more papers by this authorZhenbang Zhu, Zhenbang Zhu Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, ChinaSearch for more papers by this authorXiaohui Yao, Xiaohui Yao Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, ChinaSearch for more papers by this authorLili Yuan, Lili Yuan Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, ChinaSearch for more papers by this authorDanhe Hu, Danhe Hu Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, ChinaSearch for more papers by this authorYan Jiang, Yan Jiang Animal, Plant and Food Inspection Center of Nanjing Customs, Nanjing, ChinaSearch for more papers by this authorChanghai Chen, Corresponding Author Changhai Chen [email protected] Jiangsu Provincial Center for Animal Disease Control and Prevention, Nanjing, China Correspondence Xiangdong Li and Nanhua Chen, Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, China. Email: [email protected]; [email protected] ChanghaiChen, Jiangsu Provincial Center for Animal Disease Control and Prevention, Nanjing, China. Email: [email protected]Search for more papers by this authorNanhua Chen, Corresponding Author Nanhua Chen [email protected] orcid.org/0000-0002-1909-8760 Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, China Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou, China Correspondence Xiangdong Li and Nanhua Chen, Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, China. Email: [email protected]; [email protected] ChanghaiChen, Jiangsu Provincial Center for Animal Disease Control and Prevention, Nanjing, China. Email: [email protected]Search for more papers by this authorXiangdong Li, Corresponding Author Xiangdong Li [email protected] orcid.org/0000-0002-4635-1602 Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, China Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou, China Correspondence Xiangdong Li and Nanhua Chen, Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, China. Email: [email protected]; [email protected] ChanghaiChen, Jiangsu Provincial Center for Animal Disease Control and Prevention, Nanjing, China. Email: c[email protected]Search for more papers by this author Zhengmin Lian, Zhengmin Lian Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, China These authors contributed equally to this study.Search for more papers by this authorJinbiao Liu, Jinbiao Liu Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, China These authors contributed equally to this study.Search for more papers by this authorPanrao Liu, Panrao Liu Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, ChinaSearch for more papers by this authorZhenbang Zhu, Zhenbang Zhu Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, ChinaSearch for more papers by this authorXiaohui Yao, Xiaohui Yao Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, ChinaSearch for more papers by this authorLili Yuan, Lili Yuan Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, ChinaSearch for more papers by this authorDanhe Hu, Danhe Hu Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, ChinaSearch for more papers by this authorYan Jiang, Yan Jiang Animal, Plant and Food Inspection Center of Nanjing Customs, Nanjing, ChinaSearch for more papers by this authorChanghai Chen, Corresponding Author Changhai Chen [email protected] Jiangsu Provincial Center for Animal Disease Control and Prevention, Nanjing, China Correspondence Xiangdong Li and Nanhua Chen, Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, China. Email: [email protected]; [email protected] ChanghaiChen, Jiangsu Provincial Center for Animal Disease Control and Prevention, Nanjing, China. Email: [email protected]Search for more papers by this authorNanhua Chen, Corresponding Author Nanhua Chen [email protected] orcid.org/0000-0002-1909-8760 Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, China Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou, China Correspondence Xiangdong Li and Nanhua Chen, Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, China. Email: [email protected]; [email protected] ChanghaiChen, Jiangsu Provincial Center for Animal Disease Control and Prevention, Nanjing, China. Email: [email protected]Search for more papers by this authorXiangdong Li, Corresponding Author Xiangdong Li [email protected] orcid.org/0000-0002-4635-1602 Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, China Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou, China Correspondence Xiangdong Li and Nanhua Chen, Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, China. Email: [email protected]; [email protected] ChanghaiChen, Jiangsu Provincial Center for Animal Disease Control and Prevention, Nanjing, China. Email: [email protected]Search for more papers by this author First published: 30 July 2021 https://doi.org/10.1111/tbed.14267 Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Abstract Porcine circovirus type 4 (PCV4) was first reported in 2019 in China. So far, the viral DNA was detected from both healthy and diseased pigs in China and South Korea by using molecular techniques including PCR and real-time PCR. In contrast, a serological survey regarding the presence of PCV4 antibodies in the pig population was seldomly reported. In the present study, we describe the development of an indirect enzyme-linked immunosorbent assay (ELISA) based on capsid protein for the detection of PCV4 antibodies in swine sera. After validating the specificity and sensitivity, the ELISA was used in a retrospective serological survey for PCV4 antibodies in pig sera from Jiangsu Province of China. Note that 3.44% of analyzed samples collected between 2018 and 2021 were tested positive for PCV4 antibodies. However, PCV4 genome was absent in all ELISA-positive serum samples. Therefore, the dynamic of viremia and antibody response to PCV4 infection in pigs warrant further investigation. CONFLICT OF INTEREST The author declares no conflict of interest. Open Research DATA AVAILABILITY STATEMENT The data that support the findings of this study are available from the corresponding author upon reasonable request. Supporting Information Filename Description tbed14267-sup-0001-TableS1.docxWord document, 17.6 KB SUPPORTING INFORMATION Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume68, Issue6November 2021Pages 2975-2979 RelatedInformation