Title: Effects of dietary replacement of fish oil with soybean oil on the growth performance, plasma components, fatty acid composition and lipid metabolism of groupers <i>Epinephelus coioides</i>
Abstract: Aquaculture NutritionVolume 27, Issue 5 p. 1494-1511 ORIGINAL ARTICLE Effects of dietary replacement of fish oil with soybean oil on the growth performance, plasma components, fatty acid composition and lipid metabolism of groupers Epinephelus coioides Lingyun He, Lingyun He Xiamen Key Laboratory for Feed Quality Testing and Safety Evaluation, Fisheries College, Jimei University, Xiamen, ChinaSearch for more papers by this authorYingmei Qin, Yingmei Qin Xiamen Key Laboratory for Feed Quality Testing and Safety Evaluation, Fisheries College, Jimei University, Xiamen, ChinaSearch for more papers by this authorYanfei Wang, Yanfei Wang Xiamen Key Laboratory for Feed Quality Testing and Safety Evaluation, Fisheries College, Jimei University, Xiamen, ChinaSearch for more papers by this authorDong Li, Dong Li Xiamen Key Laboratory for Feed Quality Testing and Safety Evaluation, Fisheries College, Jimei University, Xiamen, ChinaSearch for more papers by this authorWeijun Chen, Weijun Chen Xiamen Key Laboratory for Feed Quality Testing and Safety Evaluation, Fisheries College, Jimei University, Xiamen, ChinaSearch for more papers by this authorJidan Ye, Corresponding Author Jidan Ye [email protected] orcid.org/0000-0002-6382-9604 Xiamen Key Laboratory for Feed Quality Testing and Safety Evaluation, Fisheries College, Jimei University, Xiamen, China Correspondence Jidan Ye, Fisheries College of Jimei University, Yindu Road 43, Jimei District, Xiamen 361021, China. Email: [email protected] for more papers by this author Lingyun He, Lingyun He Xiamen Key Laboratory for Feed Quality Testing and Safety Evaluation, Fisheries College, Jimei University, Xiamen, ChinaSearch for more papers by this authorYingmei Qin, Yingmei Qin Xiamen Key Laboratory for Feed Quality Testing and Safety Evaluation, Fisheries College, Jimei University, Xiamen, ChinaSearch for more papers by this authorYanfei Wang, Yanfei Wang Xiamen Key Laboratory for Feed Quality Testing and Safety Evaluation, Fisheries College, Jimei University, Xiamen, ChinaSearch for more papers by this authorDong Li, Dong Li Xiamen Key Laboratory for Feed Quality Testing and Safety Evaluation, Fisheries College, Jimei University, Xiamen, ChinaSearch for more papers by this authorWeijun Chen, Weijun Chen Xiamen Key Laboratory for Feed Quality Testing and Safety Evaluation, Fisheries College, Jimei University, Xiamen, ChinaSearch for more papers by this authorJidan Ye, Corresponding Author Jidan Ye [email protected] orcid.org/0000-0002-6382-9604 Xiamen Key Laboratory for Feed Quality Testing and Safety Evaluation, Fisheries College, Jimei University, Xiamen, China Correspondence Jidan Ye, Fisheries College of Jimei University, Yindu Road 43, Jimei District, Xiamen 361021, China. Email: [email protected] for more papers by this author First published: 05 June 2021 https://doi.org/10.1111/anu.13292Citations: 2 Lingyun He and Yingmei Qin contributed equally to this study. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract The aim of this study was to investigate the effects of substituting soybean oil (SO) for fish oil (FO) on the performance, tissue fatty acid (FA) composition, plasma components, liver metabolic enzyme activity and mRNA levels of genes related to lipid metabolism in the liver of groupers (Epinephelus coioides). We formulated five isolipidic and isoproteic diets with increasing SO levels (0, 250, 500, 750 and 1000 g/kg, respectively). Triplicate groups of 30 groupers (initial mean body weight of 12.6 g/fish) were fed one of the diets twice daily, to apparent satiety across a feeding period of 56 days. The growth performance, whole-body composition, and protein and lipid muscle contents did not differ across the dietary treatments. In contrast, the liver lipid content had positive linear and quadratic responses to the increasing dietary SO levels, but the liver protein content had the opposite trend, and the highest lipid value and lowest protein value occurred in the 250 and 1000 g/kg SO diets, respectively. There were no significant effects of increasing dietary SO inclusion levels on any of the plasma components and parameters of liver metabolic enzyme activity, except for acetyl coenzyme A carboxylase and hepatic lipase, which showed linear and quadratic responses to increasing dietary SO inclusion levels and peaked at 1000 and 250 g/kg SO inclusion level, respectively. However, the FA profiles of the dorsal muscle and liver generally reflected the FA profile of the diet. Furthermore, the mRNA levels of fas, acc, g6pd, lpl, pparα, cpt-1, srebp-1c, δ6fad and elovl5 in the liver exhibited positive linear and/or quadratic responses to dietary SO inclusion levels. However, negative linear and/or quadratic responses were observed for the mRNA levels of hsl and atgl in the liver, with increasing dietary SO inclusion levels. The mRNA levels of scd and fabp in the liver were not affected by the dietary SO inclusion level. These results indicate that FO could be replaced completely by SO without affecting growth. However, the inclusion of SO at levels higher than 500 g/kg could compromise the FA profile in the liver and flesh of the fish species. These results provide a novel insight into the potential utilization of SO in grouper feeds. CONFLICTS OF INTEREST The authors declare no conflict of interest. Open Research DATA AVAILABILITY STATEMENT The data that support the findings of this study are available from the corresponding author upon reasonable request. Citing Literature Volume27, Issue5October 2021Pages 1494-1511 RelatedInformation