Title: J‐protein requirements of multiple prion variants in two distinct yeast strains reveal functional specificity in yeast prion propagation
Abstract:The objective of this study was to determine the importance of prion variant and yeast strain variation on prion‐chaperone requirements. The J‐protein Sis1 and the prions [ PSI + ] and [ RNQ + ] were ...The objective of this study was to determine the importance of prion variant and yeast strain variation on prion‐chaperone requirements. The J‐protein Sis1 and the prions [ PSI + ] and [ RNQ + ] were used as a model system to explore the impact of amyloid conformation (prion variants) and yeast strain polymorphisms (yeast genetic background) on chaperone requirements. The primary method used in this study was complementation by plasmid‐shuffling in the budding yeast Saccharomyces cerevisiae . Summary of Results Yeast strain background had a small but noticeable effect on chaperone requirements; this effect is likely due primarily to altered chaperone protein levels between strains. While many prion variants shared similar chaperone requirements, some significant differences were found. Strikingly, despite these variations, our investigation revealed that individual prions have distinct, non‐overlapping Sis1 domain specificity, an observation which implies that Sis1 has prion‐specific functionality.Read More
Publication Year: 2013
Publication Date: 2013-04-01
Language: en
Type: article
Indexed In: ['crossref']
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