Title: ENTOMOLOGICAL PARAMETERS AND CHARACTERIZATION OF INSECTICIDE RESISTANCE IN DENGUE VECTOR AEDES AEGYPTI LARVAE FROM BENGKULU CITY, INDONESIA
Abstract: In Bengkulu, as in other areas of Indonesia, dengue is one of the most important vector-borne diseases. The primary strategy to control dengue is by reducing the vector population using insecticides. However, applications of insecticides becomes a contributing factor in the development of vector resistance. As there is no effective dengue drug or safe vaccine available, vector control remains the most effective intervention to reduce incidence and prevent transmission and outbreak. This study aimed to determine the entomological parameters and resistance status to temephos of Aedes aegypti larvae from Lingkar Barat village and Jalan Gedang village (dengue endemic areas). This study used cross-sectional and experimental with post-control only design. The sampling refers to the guidelines for dengue entomology surveys according to WHO 1999 criteria. Aedes aegypti eggs were collected by ovitraps and reared to larvae stage. Susceptibility test of larvae to the temephos was estimated using standard WHO. Mortality of larvae Ae. aegypti after 24 hours with multiple concentration of temephos indicative of highly resistant. Entomological parameters for house index (HI), container index (CI) and container pupa index (CPI) in Jalan Gedang village and Lingkar Barat village were estimated: 7%, 12% for HI and 1.97%, 3.43% for CI, and 5%, 2% for CPI, respectively. The proportion of controllable sites is higher than disposable sites, it has a high risk as breeding places for mosquitoes indicates a high potential risk as breeding place and transmission of dengue. Awareness sanitation in the home and surrounding environment must be possessed by each individual community in order to prevent and overcome the incidence of dengue. These data should be of value in formulating dengue control programs.