Title: The Optimised Statistical Model of Enzymatic Hydrolysis ofTapioca by Glucoamylase Immobilised on MesostructuredCellular Foam Silica
Abstract: Enzymatic hydrolysis of starches using free glucoamylase to produce reducing sugars,
which are substrates for various fermentation processes, often have difficulties in recovering
and recycling of the free enzyme back to the process. Therefore, free glucoamylase
immobilisation onto inert insoluble supports is widely studied. One of these supports were
siliceous-based compounds, which had large surface areas and particle diameters and could be
used for many operating cycles. However, effectiveness of glucoamylase on these supports
were observed only on soluble starches. Hence, it is considered a valuable thing to know
performance of glucoamylase immobilised on Mesostructured Cellular Foam (MCF) silica in
hydrolysing of insoluble starches such as tapioca. An optimised study on the enzymatic
tapioca hydrolysis using MCF silica (9.2T-3D)-based glucoamylase and Kinetics of the
process are described including the process justification of the predicted model.
Immobilisation of glucoamylase on this support gave up to 82% efficiency with the
immobilised enzyme specific activity of 1,856.78 U g-1. Its uses to hydrolysis of tapioca
starch resulted DE values of 1.740-76.303% (w/w) where the highest DE was obtained at pH
of 4.1, temperature of 70℃ and agitation speed of 140 rpm. The statistical optimisation
produced a polynomial quadratic model. It has insignificant lack-of-fit and low standard
deviation, so that the model applicable and reliable in simulating the results in which only
0.80% of the data were not described. Temperature affected the process highly, but the buffer
pH, agitation speed and factorial interactions were considered not important. KM value for
immobilised enzyme was better than the free glucoamylase, however, its reaction rate was
slower than the free glucoamylase catalysis.
Publication Year: 2018
Publication Date: 2018-08-15
Language: en
Type: article
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