Title: Therapeutic effects of early administration of a single high dose of rhG-CSF on mice irradiated by ~(60)Co γ rays
Abstract:Objective To observe the therapeutic effects of early administration with a single high dose of recombinant human granulocyte colony-stimulating factor(rhG-CSF) on mice irradiated with 60Co γ rays,and...Objective To observe the therapeutic effects of early administration with a single high dose of recombinant human granulocyte colony-stimulating factor(rhG-CSF) on mice irradiated with 60Co γ rays,and provide a reference for the treatment of acute radiation syndrome(ARS) by using cytokines.Methods Male C57 mice underwent a total body irradiation of 8.0Gy 60Co γ ray,and they were treated with rhG-CSF,at 0.5h and 24h,subcutaneously in a dose of 2,1 and 0.5mg/kg,respectively.The 30-day survival rate and mean survival time were observed in the lethal irradiated mice.The peripheral blood cell counts and bone marrow nucleated cell counts were evaluated in the sublethally irradiated mice.Results Early administration of a high dose of rhG-CSF significantly increased 30-day survival rate and prolonged mean survival time of mice with lethal irradiation dose.A single injection of rhG-CSF(1mg/kg) at 0.5h after irradiation was an optimal administration schedule.In addition,early administration with a single high dose of rhG-CSF improved the recovery of bone marrow nucleated cell counts and peripheral blood counts,including white blood cell(WBC),red blood cell(RBC) and platelet in mice exposed to 6.0Gy irradiation.Conclusion Early administration of a single high dose rhG-CSF may have a favorable therapeutic effect on mice irradiated with 60Co γ ray.Read More
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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