Title: A Convenient and Economical Method for Filling DNA into Microinjection Capillary
Abstract: Objective:A convenient and economical method for filling DNA into microinjection capillary was developed.Methods:Mouth pipette that was usually used for zygotes collection and transfer was modified for DNA filling.DNA solution was pipetted into a modified mouth pipette and then injected into microinjection capillary from the tail end.Results:DNA filling by present method can be finished in 2~5 min.Conclusion:Compared with traditional methods,the present is more economical and efficient,and maybe hasten the plan for development of transgenic models.
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
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