Title: Optimization of a liquid fermentation medium for Cordyceps militaris
Abstract:To obtain the optimal medium for growth of Cordyceps militaris DY-1, statistical designs were utilized on the fermentation medium.The dry weight of mycelium was measured analytically.First, the carbon...To obtain the optimal medium for growth of Cordyceps militaris DY-1, statistical designs were utilized on the fermentation medium.The dry weight of mycelium was measured analytically.First, the carbon source, nitrogen source, and inorganic salts were screened one factor at a time, and the carbon:nitrogen ratio was optimized through orthogonal testing.Then, the Plackett-Burman design was used to screen the critical factors of mycelium production.Last, a central composite design and response surface methodology were utilized to determine the optimal concentrations of the critical factors.After, the optimal medium was used in inoculation experiments to verify the predicted values.Results demonstrated that the optimal media was composed of the following factors:maltose, 16.40 g·L-1; yeast extract powder, 5.00 g·L-1; KNO3, 1.00 g·L-1; MgSO4, 0.20 g·L-1; KH2PO4, 1.80 g·L-1; and FeSO4, 0.02 g·L-1.The practical corresponding response of 32.11 g·L-1 was closer to the predicted response of 32.22 g·L-1.Using the optimum medium, the dry weight of the mycelium active substances was effectively enhanced 4.12 times compared with the initial level.The present study laid the foundation for the further research and application of Cordyceps militaris DY-1.Read More
Publication Year: 2014
Publication Date: 2014-06-20
Language: en
Type: article
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