Title: A Novel bcl 2 Antisense Oligodeoxynucleotide F951 Increases Sensitivity of HL 60 Cells to Ara C
Abstract: To investigate whether F951, a novel bcl 2 antisense oligodeoxynucleotide, increases the sensitivity of HL 60 cells to Ara C, HL 60 cells were cultured with F951 in different doses alone or with F951 combined with low dose Ara C; the proliferation of HL 60 cells was assayed by MTT and trypan blue exclusion test; expression of Bcl 2 protein and its mRNA were measured by FACS and RT PCR, respectively; the apoptotic cells were detected by DNA ladder and TUNEL assay. The results showed that F951 combined with low dose Ara C revealed stronger effects in the aspects of inhibiting the HL 60 cells proliferation than in different doses of F951 alone or Ara C alone. HL 60 cells treated with F951+Ara C had significantly lower trypan blue exclusion rate than that treated with Ara C alone. The inhibition rates of HL 60 cells treated with FNS, Ara C, F951 and F951+Ara C were -2.8%, 27.63%, 37.66%, 57.24%, respectively. F951 significantly down regulated the expression of bcl 2 mRNA and protein in HL 60 cells. HL 60 cells treated with F951 + Ara C showed more apparent DNA ladder and more apoptotic cells. It is concluded that F951 can inhibit bcl 2 gene expression and enhance the cytotoxicity of Ara C through promoting apoptosis in HL 60 cells, hence increases the antitumor effect of Ara C.
Publication Year: 2004
Publication Date: 2004-01-01
Language: en
Type: article
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